Biomedical Engineering Program, Faculty of Engineering, Chulalongkorn University, Bangkok 10330, Thailand.
Biomedical Engineering for Medical and Health Research Unit, Faculty of Engineering, Chulalongkorn University, Bangkok 10330, Thailand.
Molecules. 2021 Jun 25;26(13):3887. doi: 10.3390/molecules26133887.
Three-dimensional (3D) printing is regarded as a critical technology in material engineering for biomedical applications. From a previous report, silk fibroin (SF) has been used as a biomaterial for tissue engineering due to its biocompatibility, biodegradability, non-toxicity and robust mechanical properties which provide a potential as material for 3D-printing. In this study, SF-based hydrogels with different formulations and SF concentrations (1-3%wt) were prepared by natural gelation (SF/self-gelled), sodium tetradecyl sulfate-induced (SF/STS) and dimyristoyl glycerophosphorylglycerol-induced (SF/DMPG). From the results, 2%wt SF-based (2SF) hydrogels showed suitable properties for extrusion, such as storage modulus, shear-thinning behavior and degree of structure recovery. The 4-layer box structure of all 2SF-based hydrogel formulations could be printed without structural collapse. In addition, the mechanical stability of printed structures after three-step post-treatment was investigated. The printed structure of 2SF/STS and 2SF/DMPG hydrogels exhibited high stability with high degree of structure recovery as 70.4% and 53.7%, respectively, compared to 2SF/self-gelled construct as 38.9%. The 2SF/STS and 2SF/DMPG hydrogels showed a great potential to use as material for 3D-printing due to its rheological properties, printability and structure stability.
三维（3D）打印被认为是生物医学应用中材料工程的关键技术。根据之前的报告，丝素蛋白（SF）由于其生物相容性、可生物降解性、无毒和坚固的机械性能，已被用作组织工程的生物材料，为 3D 打印提供了潜在的材料。在这项研究中，通过天然凝胶化（SF/自凝胶化）、十四烷基硫酸钠诱导（SF/STS）和二肉豆蔻酰基甘油磷酸甘油诱导（SF/DMPG），制备了不同配方和 SF 浓度（1-3wt%）的 SF 基水凝胶。结果表明，2wt%SF 基（2SF）水凝胶具有适合挤出的特性，如储能模量、剪切稀化行为和结构恢复程度。所有 2SF 基水凝胶配方的 4 层盒结构都可以打印而不会结构坍塌。此外，还研究了三步后处理后打印结构的机械稳定性。与 2SF/自凝胶结构的 38.9%相比，2SF/STS 和 2SF/DMPG 水凝胶的打印结构具有较高的结构恢复程度，分别为 70.4%和 53.7%，表现出较高的机械稳定性。2SF/STS 和 2SF/DMPG 水凝胶由于其流变性能、可打印性和结构稳定性，具有作为 3D 打印材料的巨大潜力。
