School of Chemical Engineering, Sichuan University, Chengdu, People's Republic of China.
Biomed Mater Eng. 2022;33(2):147-158. doi: 10.3233/BME-228000.
At present, surgical resection and chemotherapy are still the main treatments for hepatocellular carcinoma and other cancers, but the curative effect and survival rate are not ideal.
In this study, we aim to prepare a carrier with low toxicity, high biocompatibility and targeted transport for the treatment of hepatocellular carcinoma.
CdSe quantum dots (QDs) modified with oleic acid were synthesized. Then hydrophobic CdSe QDs and hydrophilic super-paramagnetic Fe3O4 particles were encapsulated into different layers of liposomes to form magnetic fluorescent liposomes (MFLs). MFLs in the aqueous would quickly drift towards the external magnet and the entire process was clearly observed with fluorescence microscope. The fluorescence spectra revealed that the fluorescence properties of MFLs were similar to that of CdSe QDs.
QDs had an average size of 3.32 nm with good fluorescence properties. The size of MFLs was about 100 nm (transmission electron microscopy (TEM) analysis showed the average size of MFLs was about 82.8 nm and dynamic light scattering (DLS) detection showed 111.9 nm). After being cultured with MFLs for 8 h, HepG2 cells were labeled by MFLs, and good fluorescence images were obtained. MTT analysis also expressed their good biocompatibility.
The prepared MFLs had multi-function and could be used as ideal drug carriers.
目前，手术切除和化疗仍然是肝癌等癌症的主要治疗方法，但疗效和生存率并不理想。
本研究旨在制备一种低毒性、高生物相容性且具有靶向运输功能的载体，用于治疗肝癌。
合成了油酸修饰的 CdSe 量子点（QDs）。然后将疏水性 CdSe QDs 和亲水性超顺磁性 Fe3O4 颗粒分别包封到不同的脂质体层中，形成磁性荧光脂质体（MFLs）。在水溶液中，MFLs 会迅速向外部磁铁漂移，整个过程可以通过荧光显微镜清晰观察到。荧光光谱表明，MFLs 的荧光性质与 CdSe QDs 相似。
QDs 的平均粒径为 3.32nm，具有良好的荧光性能。MFLs 的粒径约为 100nm（透射电子显微镜（TEM）分析显示 MFLs 的平均粒径约为 82.8nm，动态光散射（DLS）检测显示为 111.9nm）。用 MFLs 培养 8 小时后，MFLs 标记 HepG2 细胞，获得良好的荧光图像。MTT 分析也表明它们具有良好的生物相容性。
制备的 MFLs 具有多功能性，可作为理想的药物载体。
