School of Medicine and Pharmacy, Ocean University of China, Qingdao, 266003, China.
Laboratory of Pathology and Immunology of Aquatic Animals, KLMME, Ocean University of China, Qingdao, 266003, China.
J Therm Biol. 2022 Feb;104:103203. doi: 10.1016/j.jtherbio.2022.103203. Epub 2022 Feb 5.
Temperature is a considerable stressor in aquatic environments. The objective of this study was to investigate the changes in endogenous compounds of flounder (Paralichthys olivaceus) at different temperatures based on a holistic metabolomic and lipidomic analysis. Accordingly, this study involved reversed-phase chromatography and hydrophilic interaction chromatography coupled with a mass spectrometry detection platform, and using a new database, and multivariate statistical analysis. In total, 954 unique masses were obtained, including 495 metabolites and 459 lipids, and 53 qualified differential metabolites were filtered. Specifically, among the highlighted biomarkers, 2'-Hydroxy-5'-methylacetophenone and FFA (17:1) were the metabolites and lipids with the largest LogFC, respectively. In addition, 18-Hydroxycorticosterone showed the greatest downregulation. Hypoxia-inducible factor 1 signaling pathway, biosynthesis of amino acids, glycerophospholipid metabolism, and choline metabolism in cancer were enriched and noteworthy metabolic pathways, which were closely related. Overall, this study provides potential serum biomarkers and metabolic pathways for flounder as well as a reference for further studies on the physiological functions of flounder under different stressors.
温度是水生环境中的一个重要应激因素。本研究旨在通过整体代谢组学和脂质组学分析，研究不同温度下牙鲆（Paralichthys olivaceus）内源性化合物的变化。为此，本研究采用反相色谱和亲水作用色谱与质谱检测平台相结合的方法，并使用新数据库和多变量统计分析。共获得 954 个独特的质量，包括 495 种代谢物和 459 种脂质，并筛选出 53 种合格的差异代谢物。具体来说，在突出的生物标志物中，2'-羟基-5'-甲基苯乙酮和 FFA（17:1）分别是代谢物和脂质中 LogFC 最大的。此外，18-羟皮质酮表现出最大的下调。缺氧诱导因子 1 信号通路、氨基酸生物合成、甘油磷脂代谢和癌症中的胆碱代谢是富集和值得注意的代谢途径，它们密切相关。总的来说，本研究为牙鲆提供了潜在的血清生物标志物和代谢途径，也为进一步研究牙鲆在不同应激因素下的生理功能提供了参考。
