DNA 错配修复蛋白、PD1 和 PDL1 在巴雷特肿瘤中的表达。
Expression of DNA Mismatch Repair Proteins, PD1 and PDL1 in Barrett's Neoplasia.
机构信息
Department of Pathology, Moffitt Cancer Center, Tampa, FL, U.S.A.
Pathology Laboratory, Florida Digestive Health Specialists, Bradenton, FL, U.S.A.
出版信息
Cancer Genomics Proteomics. 2022 Mar-Apr;19(2):145-150. doi: 10.21873/cgp.20310.
BACKGROUND/AIM: Cancers with a microsatellite instability-high (MSI-H) or deficient mismatch repair (dMMR) status respond to immune checkpoint inhibition (ICI). Regardless of the tumor type, MSI-H/dMMR status is a reliable biomarker for ICI responsiveness. This study aimed at determining the MSI-H status in precursor lesions to esophageal adenocarcinoma (EAC) such as Barrett's esophagus (BE) and BE with either low-grade dysplasia (LGD) or high-grade dysplasia (HGD).
PATIENTS AND METHODS
We performed immunohistochemical staining (IHC) for PMS2, MSH6, PD1, and PD-L1.
RESULTS
All cases of BE (50), LGD (48), and HGD (50) had intact PMS2 and MSH6 nuclear expression; were negative for PD1; and had a PD-L1 combined positive score (CPS) score <1. One EAC case (2%) was negative for PMS2 nuclear expression. One HGD case (2%) and two EAC cases (4%) were PD1 positive (CPS score <1 applied to PD1). One EAC case (2%) had a CPS score >1, and one EAC case (2%) was MSI-H. MSI-H tumors usually show PD-L1 expression, although the MSI-H EAC in this study had a PD-L1 CPS score of <1.
CONCLUSION
Further studies investigating EAC and its precursor lesions for PD1, PD-L1, and dMMR status may be informative regarding the immunogenicity of the evolution of EAC.
背景/目的：具有微卫星不稳定高（MSI-H）或错配修复缺陷（dMMR）状态的癌症对免疫检查点抑制（ICI）有反应。无论肿瘤类型如何，MSI-H/dMMR 状态都是ICI 反应性的可靠生物标志物。本研究旨在确定食管腺癌（EAC）前体病变（如 Barrett 食管（BE）和伴有低级别异型增生（LGD）或高级别异型增生（HGD）的 BE）中的 MSI-H 状态。
患者和方法
我们进行了 PMS2、MSH6、PD1 和 PD-L1 的免疫组织化学染色（IHC）。
结果
所有 BE（50 例）、LGD（48 例）和 HGD（50 例）病例均具有完整的 PMS2 和 MSH6 核表达；PD1 阴性；PD-L1 联合阳性评分（CPS）<1。1 例 EAC 病例（2%）PMS2 核表达阴性。1 例 HGD 病例（2%）和 2 例 EAC 病例（4%）PD1 阳性（PD1 的 CPS 评分<1 适用）。1 例 EAC 病例（2%）CPS 评分>1，1 例 EAC 病例（2%）MSI-H。MSI-H 肿瘤通常表现出 PD-L1 表达，尽管本研究中的 MSI-H EAC 的 PD-L1 CPS 评分<1。
结论
进一步研究 EAC 及其前体病变的 PD1、PD-L1 和 dMMR 状态可能有助于了解 EAC 进化的免疫原性。
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