Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran.
Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, 57159-44867, Urmia, Iran.
Sci Rep. 2022 Feb 18;12(1):2816. doi: 10.1038/s41598-022-06883-4.
Infertility is believed to be triggered by endometriosis whose pathophysiology and the etiology is still unknown. Certain genes play pivotal roles in pathogenesis of endometriosis. Natural products and plants are used as important sources for production of new drugs. The current study assesses the effects of gamma-oryzanol (GO) in a rat model with surgically induced endometriosis. The experimental endometriosis was induced in 24 wistar rats, and the animals were subsequently subdivided into endometriosis-sole (endom group), 3000 and 6000 µg/kg GO (GO-3000 and GO-6000) and Vit C groups. The protein levels of estrogen receptor-alpha (ER-α), Steroidogenic factor 1 (SF1), Sirtuin 1 (SIRT1), heme oxygenase 1 (HO1), light chain 3 (LC3B) and Beclin1 (BECN1) were assessed. TUNEL staining was used for detecting the apoptosis rate. The results revealed that protein levels of SF1, HO1, and total LC3B significantly (P < 0.05) decreased in GO-6000-treated groups compared to endom group. Moreover, the protein level of BECN1 and SIRT-1 significantly (P < 0.05) increased in GO-6000-treated groups compared to endom group. GO treatment did not imply any significant difference (P > 0.05) concerning the protein levels of ER-α. The TUNEL staining results showed higher TUNEL-positive cells reactions in the rats treated with GO-6000 and Vit C. Thus, GO is involved in improving condition rats involved with endometriosis through modulation in the protein levels of some molecules and also induction of apoptosis.
不孕被认为是由子宫内膜异位症引起的，其病理生理学和病因尚不清楚。某些基因在子宫内膜异位症的发病机制中起着关键作用。天然产物和植物被用作生产新药的重要来源。本研究评估了γ-谷维素（GO）在手术诱导子宫内膜异位症的大鼠模型中的作用。在 24 只 Wistar 大鼠中诱导实验性子宫内膜异位症，随后将动物分为子宫内膜异位症组（endom 组）、3000 和 6000μg/kg GO（GO-3000 和 GO-6000）和 Vit C 组。评估雌激素受体-α（ER-α）、类固醇生成因子 1（SF1）、Sirtuin 1（SIRT1）、血红素加氧酶 1（HO1）、轻链 3（LC3B）和 Beclin1（BECN1）的蛋白水平。TUNEL 染色用于检测细胞凋亡率。结果表明，与 endom 组相比，GO-6000 处理组的 SF1、HO1 和总 LC3B 蛋白水平显著降低（P<0.05）。此外，与 endom 组相比，GO-6000 处理组的 BECN1 和 SIRT-1 蛋白水平显著升高（P<0.05）。GO 处理组与 ER-α 的蛋白水平无显著差异（P>0.05）。TUNEL 染色结果显示，GO-6000 和 Vit C 处理组的 TUNEL 阳性细胞反应更高。因此，GO 通过调节某些分子的蛋白水平并诱导细胞凋亡，参与改善子宫内膜异位症大鼠的病情。
