Taguchi Ayumi, Wada-Hiraike Osamu, Kawana Kei, Koga Kaori, Yamashita Aki, Shirane Akira, Urata Yoko, Kozuma Shiro, Osuga Yutaka, Fujii Tomoyuki
Department of Obstetrics and Gynecology, Faculty of Medicine, The University of Tokyo, Tokyo, Japan.
J Obstet Gynaecol Res. 2014 Mar;40(3):770-8. doi: 10.1111/jog.12252. Epub 2013 Dec 10.
Endometriosis is a chronic inflammatory disease. Sirtuin 1 (SIRT1) plays a role in regulation of inflammation. The role of SIRT1 in endometriosis remains unknown. We here addressed the anti-inflammatory effects of SIRT1 on endometriosis.
The expression of SIRT1 in human ovarian endometriomas and eutopic endometria were examined using immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR). Endometriotic stromal cells (ESC) obtained from endometriomas were exposed to either resveratrol or sirtinol, an activator or inhibitor of sirtuins, respectively, and tumor necrosis factor (TNF)-α-induced interleukin (IL)-8 release from the ESC was assessed at mRNA and protein levels.
Both immunochemistry and RT-PCR demonstrated that SIRT1 was expressed in ESC and normal endometrial stromal cells. Resveratrol suppressed TNF-α-induced IL-8 release from the ESC in a dose-dependent manner while sirtinol increased IL-8 release.
These opposing effects of SIRT1-related agents suggest that IL-8 release from the ESC is modulated through the SIRT1 pathway. Resveratrol may have the potential to ameliorate local inflammation in endometriomas.
子宫内膜异位症是一种慢性炎症性疾病。沉默调节蛋白1（SIRT1）在炎症调节中发挥作用。SIRT1在子宫内膜异位症中的作用尚不清楚。我们在此探讨SIRT1对子宫内膜异位症的抗炎作用。
采用免疫组织化学和逆转录聚合酶链反应（RT-PCR）检测人卵巢子宫内膜异位囊肿和在位子宫内膜中SIRT1的表达。从子宫内膜异位囊肿中获取的异位子宫内膜间质细胞（ESC）分别用白藜芦醇或瑟土因激活剂或抑制剂西曲瑞克处理，并在mRNA和蛋白质水平评估肿瘤坏死因子（TNF）-α诱导的ESC中白细胞介素（IL）-8的释放。
免疫化学和RT-PCR均表明SIRT1在ESC和正常子宫内膜间质细胞中表达。白藜芦醇以剂量依赖的方式抑制TNF-α诱导的ESC中IL-8的释放，而西曲瑞克则增加IL-8的释放。
SIRT1相关药物的这些相反作用表明，ESC中IL-8的释放是通过SIRT1途径调节的。白藜芦醇可能具有改善子宫内膜异位囊肿局部炎症的潜力。
