Guan Tian, Xu Zhenlin, Wang Jin, Liu Yingju, Shen Xing, Li Xiangmei, Sun Yuanming, Lei Hongtao
Guangdong Provincial Key Laboratory of Food Quality and Safety / Nation-Local Joint Engineering Research Center for Machining and Safety of Livestock and Poultry Products, College of Food Science, South China Agricultural University, Guangzhou, 510642, China.
Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, China.
Compr Rev Food Sci Food Saf. 2022 Mar;21(2):1627-1656. doi: 10.1111/1541-4337.12914. Epub 2022 Feb 18.
Food safety analysis plays a significant role in controlling food contamination and supervision. In recent years, multiplex optical bioassays (MOBAs) have been widely applied to analyze multiple hazards due to their efficiency and low cost. However, due to the challenges such as multiplexing capacity, poor sensitivity, and bulky instrumentation, the further application of traditional MOBAs in food screening has been limited. In this review, effective strategies regarding food safety MOBAs are summarized, such as spatial-resolution modes performed in multi-T lines/dots strips or arrays of strip/microplate/microfluidic chip/SPR chip and signal-resolution modes employing distinguishable colorimetric/luminescence/fluorescence/surface plasma resonance/surface-enhanced Raman spectrum as signal tags. Following this, new trends on how to design engineered sensor architecture and exploit distinguishable signal reporters, how to improve both multiplexing capacity and sensitivity, and how to integrate these formats into smartphones so as to be mobile are summarized systematically. Typically, in the case of enhancing multiplexing capacity and detection throughput, microfluidic array chips with multichannel architecture would be a favorable approach to overcome the spatial and physical limitations of immunochromatographic assay (ICA) test strips. Moreover, noble metal nanoparticles and single-excitation, multiple-emission luminescence nanomaterials hold great potential in developing ultrasensitive MOBAs. Finally, the exploitation of innovative multiplexing strategy hybridized with powerful and widely available smartphones opens new perspectives to MOBAs. In future, the MOBAs should be more sensitive, have higher multiplexing capacity, and easier instrumentation.
食品安全分析在控制食品污染和监管方面发挥着重要作用。近年来，多重光学生物分析技术（MOBAs）因其高效性和低成本，已被广泛应用于多种危害因素的分析。然而，由于存在诸如复用能力有限、灵敏度差以及仪器设备笨重等挑战，传统的MOBAs在食品筛查中的进一步应用受到了限制。在这篇综述中，总结了有关食品安全MOBAs的有效策略，例如在多T线/点试纸条或试纸条/微孔板/微流控芯片/表面等离子体共振（SPR）芯片阵列中执行的空间分辨率模式，以及采用可区分的比色/发光/荧光/表面等离子体共振/表面增强拉曼光谱作为信号标签的信号分辨率模式。在此之后，系统地总结了关于如何设计工程化传感器架构和开发可区分信号报告器、如何提高复用能力和灵敏度，以及如何将这些形式集成到智能手机中以实现移动性的新趋势。通常情况下，在提高复用能力和检测通量方面，具有多通道架构的微流控阵列芯片将是克服免疫层析分析（ICA）试纸条空间和物理限制的有利方法。此外，贵金属纳米颗粒以及单激发、多发射发光纳米材料在开发超灵敏MOBAs方面具有巨大潜力。最后，与功能强大且广泛可用的智能手机相结合的创新复用策略的开发为MOBAs开辟了新的前景。未来，MOBAs应更加灵敏、具有更高的复用能力且仪器设备更简便。
