Fan Wujiang, Yang Pinhong, Qiao Yanping, Su Ming, Zhang Genyu
Shanghai Fisheries Research Institute (Shanghai Fisheries Technology Promotion Station), Shanghai, 200433, China; Hunan University of Arts and Science, Hunan, 41500, China.
Hunan University of Arts and Science, Hunan, 41500, China.
Fish Shellfish Immunol. 2022 Mar;122:419-425. doi: 10.1016/j.fsi.2022.02.028. Epub 2022 Feb 16.
The widespread occurrence and accumulation of plastic waste have been globally recognized as a critical issue. However, few researches have evaluated the adverse effects of nanoplastics to freshwater organisms. Thus, here, the effects of polystyrene nanoplastics (PS-NP) on the physiological changes (i.e., molting) and enzyme activity of oxidative stress were investigated in the adult freshwater prawn Macrobrachium nipponense. Based on a previous study and environmental microplastic concentrations, the prawn was exposed to 0, 0.04, 0.4, 4, and 40 mg/L waterborne PS-NP for 21 days. The results showed that growth and survival-related parameters were not affected by all PS-NP groups, while the molting rate were significantly decreased in the 4 and 40 mg/L PS-NP group. Meanwhile, the expression of molting-related gene (calcium-calmodulin-dependent protein kinase I, ecdysteroid receptor, and leucine-rich repeat-containing G-protein-coupled receptor 2) were significantly decreased. HO content was significantly increased in all PS-NP groups relative to the control. Lower concentrations of PS-NP increased the activity of superoxide dismutase (SOD), glutathione S-transferase (GST), and glutathione peroxidase (GSH-Px), whereas increased concentrations, decreased SOD, GST, and GSH-Px activity. These results suggest that chronic exposure to PS-NP at an environmental concentration impaired molting and induced oxidative stress in the adult river prawn Macrobrachium nipponense. The findings provided basic information for assessing the risk assessment of nanoplastics and revealing the molecular mechanisms of nanoplastics toxicity.
塑料垃圾的广泛存在和积累已被全球公认为一个关键问题。然而，很少有研究评估纳米塑料对淡水生物的不利影响。因此，在此研究中，我们调查了聚苯乙烯纳米塑料（PS-NP）对成年淡水虾日本沼虾生理变化（即蜕皮）和氧化应激酶活性的影响。根据先前的研究和环境微塑料浓度，将虾暴露于0、0.04、0.4、4和40mg/L的水体PS-NP中21天。结果表明，所有PS-NP组的生长和存活相关参数均未受到影响，而4和40mg/L PS-NP组的蜕皮率显著降低。同时，蜕皮相关基因（钙调蛋白依赖性蛋白激酶I、蜕皮甾类受体和富含亮氨酸重复序列的G蛋白偶联受体2）的表达显著降低。相对于对照组，所有PS-NP组的HO含量均显著增加。较低浓度的PS-NP增加了超氧化物歧化酶（SOD）、谷胱甘肽S-转移酶（GST）和谷胱甘肽过氧化物酶（GSH-Px）的活性，而浓度增加则降低了SOD、GST和GSH-Px的活性。这些结果表明，在环境浓度下长期暴露于PS-NP会损害成年河虾日本沼虾的蜕皮并诱导氧化应激。这些发现为评估纳米塑料的风险评估和揭示纳米塑料毒性的分子机制提供了基础信息。
