Virtanen I, Kallajoki M, Närvänen O, Paranko J, Thornell L E, Miettinen M, Lehto V P
Anat Rec. 1986 May;215(1):10-20. doi: 10.1002/ar.1092150103.
We studied the cytoskeletal composition of human and rat testicular myoid cells by using immunofluorescence microscopy with polyclonal and monoclonal antibodies. In adult human and rat testis, the peritubular myoid cell layer was brightly positive for desmin, the muscle type of intermediate filament protein, and a faint reaction was also seen with antibodies to vimentin, the intermediate filament protein of fibroblasts and diverse other mesenchymal cells. The desmin-positive myoid cell layer could already be identified in newborn rat testis but was more compact in appearance 23 days after birth. Both squash preparations and cultured cells from adult rat seminiferous tubules revealed distinct cell populations positive for desmin. The adult myoid cells of both species also showed a strong reaction with antibodies to myosin and p230, a nonerythroid avian alpha-spectrin analogue. The immunostaining results could be confirmed by the western blotting technique: Experiments with isolated seminiferous tubules showed a specific reaction with a 55,000-dalton and a 58,000-dalton polypeptide when desmin and vimentin antibodies were used, respectively. The present results show that the peritubular myoid cells are genuine smooth muscle cells with desmin-type intermediate filament cytoskeleton and suggest that these cells can be identified by this feature before their ultrastructural maturation.
我们通过使用针对多克隆和单克隆抗体的免疫荧光显微镜技术，研究了人和大鼠睾丸肌样细胞的细胞骨架组成。在成年人类和大鼠的睾丸中，生精小管周围的肌样细胞层对结蛋白呈强阳性反应，结蛋白是肌型中间丝蛋白，而对波形蛋白（成纤维细胞及其他多种间充质细胞的中间丝蛋白）的抗体也呈现微弱反应。在新生大鼠睾丸中就已能识别出结蛋白阳性的肌样细胞层，但在出生23天后其外观更为紧密。成年大鼠生精小管的压片标本和培养细胞均显示出结蛋白阳性的不同细胞群体。这两个物种的成年肌样细胞对肌球蛋白和p230（一种非红细胞禽类α-血影蛋白类似物）的抗体也呈现强烈反应。免疫染色结果可通过蛋白质印迹技术得到证实：对分离的生精小管进行的实验表明，当分别使用结蛋白和波形蛋白抗体时，会与一条55,000道尔顿和一条58,000道尔顿的多肽发生特异性反应。目前的结果表明，生精小管周围的肌样细胞是具有结蛋白型中间丝细胞骨架的真正平滑肌细胞，并提示这些细胞在超微结构成熟之前就可通过这一特征得以识别。
