Ren Shuaijun, Wang Wenjiao, Jia Min, An Lili, Tang Ting, Liu Xin
Guizhou University of Traditional Chinese Medicine, No. 50, Shidong Rd., Nanming District, Guiyang City, Guizhou Province 550001, China.
Dermatology Department, The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, No. 71, Baoshan North Rd., Yunyan District, Guiyang City, Guizhou Province 550002, China.
Evid Based Complement Alternat Med. 2022 Feb 9;2022:3855702. doi: 10.1155/2022/3855702. eCollection 2022.
is a major pathogen of acne vulgaris. The traditional Chinese medicine (TCM) compound prescription, Dian Dao San (DDS), is effective for treating . Previous clinical work by our team demonstrated that improved Dian Dao San (IDDS) has better antibacterial effects. However, the mechanism of IDDS inhibition of is still unknown. Hence, the isobaric tags for relative and absolute quantitation (iTRAQ) technology was applied to explore the antibacterial mechanism of IDDS against Our results suggested that the antibacterial mechanism of IDDS was related to the glycolytic pathway. gap, pgk, and tpiA enzymes were found to be potential target proteins in the bacterial glycolytic pathway as an antibacterial mechanism of inhibition. In addition, SEM and TEM analyses revealed that IDDS may destruct bacterial plasma membrane and cell wall. The results provide a reliable, direct, and scientific theoretical basis for wide application of IDDS.
是寻常痤疮的主要病原菌。中药复方颠倒散（DDS）对治疗有效。我们团队之前的临床工作表明，改良颠倒散（IDDS）具有更好的抗菌效果。然而，IDDS抑制的机制仍不清楚。因此，应用相对和绝对定量等压标签（iTRAQ）技术探讨IDDS对的抗菌机制。我们的结果表明，IDDS的抗菌机制与糖酵解途径有关。发现gap、pgk和tpiA酶是细菌糖酵解途径中作为抗菌抑制机制的潜在靶蛋白。此外，扫描电子显微镜（SEM）和透射电子显微镜（TEM）分析表明，IDDS可能破坏细菌的质膜和细胞壁。这些结果为IDDS的广泛应用提供了可靠、直接和科学的理论依据。
