An Lili, Gong Nan, Hu Taoting, Wang Lan, Zhang Mei, Huang Minjia, Chen Gongzhen, Tang Ting, Liu Xin
Guizhou University of Traditional Chinese Medicine, Guiyang City, People's Republic of China.
Dermatology Department, The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang City, People's Republic of China.
Infect Drug Resist. 2023 Aug 1;16:4965-4975. doi: 10.2147/IDR.S419161. eCollection 2023.
The hyperproliferation of has long been regarded as a primary etiological factor in the development of acne vulgaris (AV). Antibiotics targeting have been the mainstay in AV treatment. Meanwhile, has developed resistance to numerous antibiotics. IDDS, as traditional Chinese medicine, exhibits potent antibacterial activity against . However, the mechanism of IDDS against remains unclear.
In this study, we conducted a systematic investigation in vitro to determine the minimal bactericidal concentration (MBC) and time-kill curves. The MBC and time-kill curves were assessed by quantifying Colony Forming Units countsIn order to establish an in vivo rat ear model of acne, a single intradermal injection of 100μL suspension was administered, and oleic acid was applied to the right ear pinna for a duration of 14 days. The intervention involved the utilization of IDDS medications. Additionally, the levels of inflammatory mediators tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-10 (IL-10) were assessed using respective ELISA kits, while Hematoxylin and eosin (HE) staining was employed to visualize the rat ear model. The antimicrobial mechanism was investigated through the analysis of mRNA levels using real-time, quantitative PCR. ELISA analysis was performed according to the protocols outlined for energy metabolism and antioxidant system.
Our research has demonstrated that IDDS possesses antibacterial activity against both in vitro and in vivo. The mechanisms underlying these effects involve energy metabolism and antioxidant systems.
The data has provided further insights into the mechanism of IDDS against , which establishes a robust foundation for the clinical application of IDDS.
长期以来，[细菌名称未给出]的过度增殖一直被视为寻常痤疮（AV）发病的主要病因。针对[细菌名称未给出]的抗生素一直是AV治疗的主要手段。与此同时，[细菌名称未给出]已对多种抗生素产生耐药性。IDDS作为传统中药，对[细菌名称未给出]具有强大的抗菌活性。然而，IDDS抗[细菌名称未给出]的机制仍不清楚。
在本研究中，我们进行了体外系统研究以确定最低杀菌浓度（MBC）和时间 - 杀菌曲线。通过定量菌落形成单位计数来评估MBC和时间 - 杀菌曲线。为建立痤疮大鼠耳模型，皮内注射100μL[细菌悬液名称未给出]悬液，并在右耳廓涂抹油酸，持续14天。干预措施包括使用IDDS药物。此外，使用相应的ELISA试剂盒评估炎症介质肿瘤坏死因子 -α（TNF -α）、白细胞介素 -6（IL -6）和白细胞介素 -10（IL -10）的水平，同时采用苏木精和伊红（HE）染色来观察大鼠耳模型。通过实时定量PCR分析mRNA水平来研究抗菌机制。ELISA分析按照能量代谢和抗氧化系统概述的方案进行。
我们的研究表明，IDDS在体外和体内均对[细菌名称未给出]具有抗菌活性。这些作用的潜在机制涉及能量代谢和抗氧化系统。
这些数据为IDDS抗[细菌名称未给出]的机制提供了进一步的见解，为IDDS的临床应用奠定了坚实的基础。
