Mayo K E, Evans R M, Rosenfeld G M
Annu Rev Physiol. 1986;48:431-46. doi: 10.1146/annurev.ph.48.030186.002243.
During the last five years, cDNAs and genes encoding more than 20 neuroendocrine peptides have been cloned. The information gleaned from these genes has been largely structural to this point, and illustrates the great versatility of neuroendocrine genes in generating diverse sets of bioactive peptides. An ongoing contribution of the application of recombinant DNA techniques to the neuroendocrine system promises to be the identification of novel peptides; it will require a concerted effort to determine their physiological functions. The application of recombinant DNA to the study of expression and regulation of neuroendocrine genes is still in its infancy, but a variety of newly emerging techniques promise to be useful for these approaches. Although we have considered the application of recombinant DNA only to the cloning and analysis of neuroendocrine peptide genes, molecular biology will clearly also have a major future impact in investigating aspects of other molecules involved in neuropeptide action, such as processing enzymes and peptide receptors.
在过去五年中，编码20多种神经内分泌肽的cDNA和基因已被克隆。到目前为止，从这些基因中收集到的信息主要是关于结构方面的，这说明了神经内分泌基因在产生多种生物活性肽方面具有很大的通用性。将重组DNA技术应用于神经内分泌系统的一个持续贡献有望是鉴定新的肽；确定它们的生理功能需要共同努力。重组DNA在神经内分泌基因表达和调控研究中的应用仍处于起步阶段，但各种新出现的技术有望对这些方法有用。虽然我们只考虑了重组DNA在神经内分泌肽基因克隆和分析中的应用，但分子生物学显然在研究参与神经肽作用的其他分子方面，如加工酶和肽受体，也将在未来产生重大影响。
