Helman L J, Gazdar A F, Park J G, Cohen P S, Cotelingam J D, Israel M A
Naval Medical Oncology Branch, National Cancer Institute, Bethesda, Maryland 20892.
J Clin Invest. 1988 Aug;82(2):686-90. doi: 10.1172/JCI113648.
We used a recombinant cDNA probe for human chromogranin A to measure the expression of mRNA encoded by this gene in a variety of normal human tissues and tumor specimens using Northern blot and in situ hybridization analysis. With few exceptions, the expression of chromogranin A mRNA appears to be restricted to normal tissues and tumors of neuroendocrine lineage. However, we have detected mRNA expression of this gene in 1 of 14 cell lines and 2 of 13 tumor specimens of colon adenocarcinoma. The finding of chromogranin A expression in some colon carcinomas suggests that a previously unrecognized subgroup of these tumors has neuroendocrine features. The detection of this subgroup demonstrates the potential for improving tumor classification through the use of techniques and reagents developed by recombinant DNA technology.
我们使用人嗜铬粒蛋白A的重组cDNA探针，通过Northern印迹和原位杂交分析，来检测该基因编码的mRNA在多种正常人体组织和肿瘤标本中的表达。除少数例外情况外，嗜铬粒蛋白A mRNA的表达似乎仅限于神经内分泌谱系的正常组织和肿瘤。然而，我们在14个细胞系中的1个以及13个结肠腺癌肿瘤标本中的2个中检测到了该基因的mRNA表达。在某些结肠癌中发现嗜铬粒蛋白A表达，表明这些肿瘤中一个先前未被认识的亚组具有神经内分泌特征。该亚组的检测证明了通过使用重组DNA技术开发的技术和试剂来改善肿瘤分类的潜力。
