Swaminathan S, Chandrasekher G, Venkataraman A, Pattabiraman T N
Biochem Med Metab Biol. 1986 Apr;35(2):184-90. doi: 10.1016/0885-4505(86)90073-3.
Trypsin inhibition (reduction in benzoyl arginine p-nitroanilide hydrolysis), elastase inhibition (reduction in succinyl trialanyl p-nitroanilide hydrolysis), and chymotrypsin inhibition (reduction in acetyl tyrosine ethyl ester hydrolysis) by neutral extracts of mammalian lenses were estimated. The activities were found to be markedly elevated in human cortical cataract lenses compared to normal adult lenses (antielastase 7.21 +/- 3.90 units (mean +/- SD) in cataract compared to 1.46 +/- 0.57 in normals; antitryptic, 0.54 +/- 0.38 and 0.12 +/- 0.04; antichymotryptic, 1.03 +/- 0.61 and 0.297 +/- 0.055). Antielastase activity was distinctly higher in adult normal human lenses compared to infant lenses (0.159 +/- 0.068). Elastase- and trypsin-like activities were detected at low levels in all mammalian lenses. Chymotrypsin-like activity could not be observed in the lenses. The cataractous lenses had lower trypsin- and elastase-like activities compared to normal human lenses (elastase 1.20 +/- 0.643 in normal compared to 0.062 +/- 0.035 in cataract; trypsin, 0.367 +/- 0.154 and 0.069 +/- 0.038). The role of protease: inhibitor complexes in the expression of the individual activities and their role in cataractogenesis are discussed.
对哺乳动物晶状体中性提取物的胰蛋白酶抑制作用（对苯甲酰精氨酸对硝基苯胺水解的抑制）、弹性蛋白酶抑制作用（对琥珀酰丙氨酰对硝基苯胺水解的抑制）和糜蛋白酶抑制作用（对乙酰酪氨酸乙酯水解的抑制）进行了评估。结果发现，与正常成人晶状体相比，人类皮质性白内障晶状体中的这些活性显著升高（白内障患者的抗弹性蛋白酶活性为7.21±3.90单位（平均值±标准差），而正常人的为1.46±0.57；抗胰蛋白酶活性分别为0.54±0.38和0.12±0.04；抗糜蛋白酶活性分别为1.03±0.61和0.297±0.055）。与婴儿晶状体相比，成人正常人类晶状体中的抗弹性蛋白酶活性明显更高（0.159±0.068）。在所有哺乳动物晶状体中均检测到低水平的弹性蛋白酶样和胰蛋白酶样活性。在晶状体中未观察到糜蛋白酶样活性。与正常人类晶状体相比，白内障晶状体中的胰蛋白酶样和弹性蛋白酶样活性较低（正常晶状体中的弹性蛋白酶活性为1.20±0.643，而白内障晶状体中的为0.062±0.035；胰蛋白酶活性分别为0.367±0.154和0.069±0.038）。讨论了蛋白酶：抑制剂复合物在个体活性表达中的作用及其在白内障发生中的作用。
