Kidron M, Dudai M, Nachshon I, Mayer M
Biochem Med Metab Biol. 1986 Oct;36(2):136-40. doi: 10.1016/0885-4505(86)90117-9.
Myofibrillar protease activity and activity of inhibitors toward trypsin, chymotrypsin, elastase, and the myofibrillar protease were determined in human skeletal muscle. The protease activity was found to increase in patients with acute and chronic inflammation as well as in patients with metastatic and nonmetastatic tumors. The inhibitory activity directed against trypsin and chymotrypsin was not affected by acute inflammation, while the inhibition of elastase and the myofibrillar protease was increased. Chronic inflammation did not affect the ability of the muscle cytosol to inhibit trypsin and elastase, but increased the inhibition of chymotrypsin and the myofibrillar protease. Nonmetastatic tumors produced an increase in the activity of inhibitors toward trypsin, chymotrypsin, and elastase, while patients bearing metastatic tumors had a high level of cytosolic inhibitors of all the tested proteolytic activities. These results indicate that the myofibrillar protease and the cytosolic protease inhibitors in human skeletal muscle are differentially affected by catabolic conditions.
在人体骨骼肌中测定了肌原纤维蛋白酶活性以及其对胰蛋白酶、胰凝乳蛋白酶、弹性蛋白酶和肌原纤维蛋白酶的抑制剂活性。结果发现，急性和慢性炎症患者以及转移性和非转移性肿瘤患者的蛋白酶活性均升高。针对胰蛋白酶和胰凝乳蛋白酶的抑制活性不受急性炎症影响，而对弹性蛋白酶和肌原纤维蛋白酶的抑制作用增强。慢性炎症不影响肌肉胞质溶胶抑制胰蛋白酶和弹性蛋白酶的能力，但增强了对胰凝乳蛋白酶和肌原纤维蛋白酶的抑制作用。非转移性肿瘤使对胰蛋白酶、胰凝乳蛋白酶和弹性蛋白酶的抑制剂活性增加，而患有转移性肿瘤的患者对所有测试的蛋白水解活性均有高水平的胞质溶胶抑制剂。这些结果表明，人体骨骼肌中的肌原纤维蛋白酶和胞质溶胶蛋白酶抑制剂受分解代谢状况的影响存在差异。
