Santoro Domenico, Archer Linda, Chong Eric
Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL, USA.
Vet Dermatol. 2022 Jun;33(3):195-e56. doi: 10.1111/vde.13061. Epub 2022 Feb 20.
Canine atopic dermatitis (AD) is a complex multifactorial disease characterised by an exaggerated immunological response. Little is known about the role that cutaneous and circulating chemokines play in disease severity.
To evaluate the messenger (m)RNA and protein levels of selected chemokines in skin and serum of healthy and atopic dogs, and in the atopic group to determine whether there is a correlation with disease severity.
Skin biopsies and blood samples were taken from 12 privately owned atopic [lesional (AD-L) and nonlesional (AD-NL) skin] and 12 privately owned healthy dogs. Circulating exosomes were extracted from the serum. Cutaneous and exosomal mRNA levels of CCL17, CCL22, CCL27 and CCL28 were quantified using quantitative real-time PCR. Protein levels were evaluated using canine-specific ELISA kits. The severity and extent of the clinical signs also were assessed in the atopic dogs using Canine Atopic Dermatitis Extent and Severity Index, 4 iteration (CADESI-04) and a validated pruritus Visual Analog Scale (pVAS).
The expression of CCL28 exosomes in skin was greater in AD-L when compared to healthy (P = 0.019) and AD-NL (P = 0.002) samples. However, serum expression was lower in dogs with AD compared to healthy dogs (P = 0.03). A higher expression of CCL17 and CCL22 was seen in AD-L when compared to healthy skin (P = 0.018 and P = 0.019, respectively). There also was a positive correlation between clinical scores and CCL22 (AD-NL; r = 0.6, P = 0.05) and between the pruritus score and CCL22 (AD-L; r = 0.6, P = 0.05). Differences in CCL27 concentrations were not observed.
This study suggests that CCL17, CCL22 and CCL28 may play a role in the cutaneous inflammatory response in atopic dogs. They may be considered as markers of disease severity, although further studies are needed to validate these findings.
犬特应性皮炎（AD）是一种复杂的多因素疾病，其特征为免疫反应过度。关于皮肤和循环趋化因子在疾病严重程度中所起的作用，人们了解甚少。
评估健康犬和特应性犬的皮肤及血清中选定趋化因子的信使核糖核酸（mRNA）和蛋白质水平，并在特应性犬组中确定其与疾病严重程度是否存在相关性。
从12只 privately owned 特应性犬（有病变皮肤（AD-L）和无病变皮肤（AD-NL））和12只 privately owned 健康犬身上采集皮肤活检样本和血液样本。从血清中提取循环外泌体。使用定量实时聚合酶链反应（PCR）对CCL17、CCL22、CCL27和CCL28的皮肤及外泌体mRNA水平进行定量。使用犬特异性酶联免疫吸附测定（ELISA）试剂盒评估蛋白质水平。还使用犬特应性皮炎范围和严重程度指数第4版（CADESI-04）和经过验证的瘙痒视觉模拟量表（pVAS）对特应性犬的临床症状严重程度和范围进行评估。
与健康样本（P = 0.019）和AD-NL样本（P = 0.002）相比，AD-L皮肤中CCL28外泌体的表达更高。然而，与健康犬相比，患有AD的犬血清表达较低（P = 0.03）。与健康皮肤相比，AD-L中CCL17和CCL22的表达更高（分别为P = 0.018和P = 0.019）。临床评分与CCL22之间（AD-NL；r = 0.6，P = 0.05）以及瘙痒评分与CCL22之间（AD-L；r = 0.6，P = 0.05）也存在正相关。未观察到CCL27浓度的差异。
本研究表明CCL17、CCL22和CCL28可能在特应性犬的皮肤炎症反应中起作用。它们可被视为疾病严重程度的标志物，尽管需要进一步研究来验证这些发现。
privately owned 这里推测可能是“私人拥有的”意思，但在专业医学语境中也许有特定含义，因信息有限无法准确翻译其确切含义。
