Faculdade de Ciências Biológicas e Saúde (FCBS), Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM); Programa de Pós-Graduação em Multicêntrico em Ciências Fisiológicas (PMPGCF), Sociedade Brasileira de Fisiologia (SBFis); Grupo de Estudos em Neurociências e Exercício (GENE), Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM).
Faculdade de Ciências Biológicas e Saúde (FCBS), Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM); Programa de Pós-Graduação em Multicêntrico em Ciências Fisiológicas (PMPGCF), Sociedade Brasileira de Fisiologia (SBFis).
J Vis Exp. 2022 Feb 2(180). doi: 10.3791/63328.
High-Intensity Interval Training (HIIT) and accumulated exercises are two time-efficient programs to improve health in humans and animal models. However, to date, there are no studies on whether HIIT performed in an accumulated fashion is as effective as a traditional HIIT performed with single daily sessions in improving health markers. This paper presents the effects of a new HIIT protocol, called accumulated HIIT, on body weight gain, maximal oxygen consumption (VO2max), and cardiac hypertrophy in young Wistar rats. Sixty-day-old male Wistar rats were assigned to three groups: untrained (UN; n = 16), HIIT performed with single daily sessions (1-HIIT; n = 16), and HIIT performed with three daily sessions (3-HIIT; n = 16). Body weight and VO2max were recorded before and after the training period. The VO2max measurements were taken using a metabolic analyzer at the maximal running velocity (Vmax). The training was performed for both HIIT groups five days per week over eight weeks with the same weekly progression of the exercise intensity (85-100% Vmax). The 1-HIIT group performed single daily sessions (6 bouts of 1 min interspersed with 1 min of passive recovery). The 3-HIIT group performed three daily sessions (2 bouts of 1 min interspersed with 1 min of passive recovery with an interval of 4 h between bouts). After the last VO2max test, the rats were euthanized, and their hearts were harvested and weighed. The results showed that 3-HIIT had similar beneficial effects to 1-HIIT in preventing body weight gain, improving VO2max, and inducing cardiac hypertrophy. These findings reveal for the first time the efficacy of an accumulated HIIT protocol on the health markers of young Wistar rats. This new HIIT protocol may be more feasible than traditional HIIT protocols as exercise can be split into very short sessions throughout a day in this new approach.
高强度间歇训练（HIIT）和累积运动是两种提高人类和动物模型健康的高效时间方案。然而，迄今为止，尚无研究表明以累积方式进行的 HIIT 是否与传统的每日单次 HIIT 一样有效，可改善健康标志物。本文介绍了一种新的 HIIT 方案，称为累积 HIIT，对年轻 Wistar 大鼠体重增加、最大摄氧量（VO2max）和心脏肥大的影响。将 60 天大的雄性 Wistar 大鼠分为三组：未训练组（UN；n = 16）、每日单次 HIIT 组（1-HIIT；n = 16）和每日三次 HIIT 组（3-HIIT；n = 16）。在训练期前后记录体重和 VO2max。使用代谢分析仪在最大跑步速度（Vmax）下测量 VO2max。训练是针对两个 HIIT 组进行的，每周进行五天，每周的运动强度呈递增趋势（85-100% Vmax）。1-HIIT 组进行每日单次训练（6 个 1 分钟的回合，间隔 1 分钟的被动恢复）。3-HIIT 组进行每日三次训练（2 个 1 分钟的回合，间隔 1 分钟的被动恢复，回合之间间隔 4 小时）。最后一次 VO2max 测试后，处死大鼠，取出心脏称重。结果表明，3-HIIT 对预防体重增加、提高 VO2max 和诱导心脏肥大具有与 1-HIIT 相似的有益作用。这些发现首次揭示了累积 HIIT 方案对年轻 Wistar 大鼠健康标志物的疗效。与传统的 HIIT 方案相比，这种新的 HIIT 方案可能更可行，因为在这种新方法中，运动可以分成全天非常短的时间段进行。
