Chen Ruiqi, Qin Shuang, Zhu Hengyue, Chang Guolin, Li Meihui, Lu Hong, Shen Mo, Gao Qiuqi, Lin Xiangyang
Department of Clinical Laboratory, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China.
School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, 325000, China.
Immunol Lett. 2022 Mar;243:61-68. doi: 10.1016/j.imlet.2022.02.004. Epub 2022 Feb 19.
Septic shock is a great threat to human life. Our aim is to explore the immune status and dynamic changes of circulating cytotoxic cells in septic shock patients.
Forty-eight septic shock patients (9 non-survivors and 39 survivors) and 30 healthy controls (HCs) were enroled in our study. The function of cytotoxic cells was dynamically monitored by flow cytometry.
The number of circulating CD8 T and NK cells decreased significantly in septic shock patients, while the number of CD8 T cells rose in survivors 5 days after admission. The frequency of HLA-DRCD8 T/ NK cells increased in both groups after admission but decreased in non-survivors on day 3. Moreover, the frequency of GrA/GrB/perforinNK and GrBCD8 T cells decreased to varying degrees in both groups, and the frequency of GrB/perforinCD8 T cells on the second day of non-survivors was significantly lower than that of survival patients. Besides, the frequency of CXCR3CD8 T/ NK cells was decreased in both groups and remained low in non-survivors, but remarkably increased in survivors after day 3. And the concentrations of cytokines IL-6, IL-10, TNF-α and IFN-γ were significantly increased in septic shock patients.
Circulating CD8 T and NK cells reduced but activation function was compensatory enhanced in septic shock patients. The frequency of GrB/PFPCD8 T and CXCR3CD8 T/NK cells may predict the progression of septic shock patients 2-3 days after admission.
感染性休克对人类生命构成巨大威胁。我们的目的是探讨感染性休克患者循环细胞毒性细胞的免疫状态及动态变化。
本研究纳入了48例感染性休克患者（9例非幸存者和39例幸存者）和30例健康对照者（HCs）。通过流式细胞术动态监测细胞毒性细胞的功能。
感染性休克患者循环CD8 T细胞和NK细胞数量显著减少，而幸存者入院5天后CD8 T细胞数量上升。两组患者入院后HLA-DR⁺CD8 T/NK细胞频率均增加，但非幸存者在第3天下降。此外，两组患者GrA/GrB/穿孔素⁺NK细胞和GrB⁺CD8 T细胞频率均有不同程度下降，非幸存者第2天GrB/穿孔素⁺CD8 T细胞频率显著低于存活患者。此外，两组患者CXCR3⁺CD8 T/NK细胞频率均下降，非幸存者持续处于低水平，但幸存者在第3天后显著增加。感染性休克患者细胞因子IL-6、IL-10、TNF-α和IFN-γ浓度显著升高。
感染性休克患者循环CD8 T细胞和NK细胞减少，但激活功能呈代偿性增强。GrB/PFP⁺CD8 T细胞和CXCR3⁺CD8 T/NK细胞频率可能预测感染性休克患者入院后2 - 3天的病情进展。
