Bacârea Anca, Coman Oana, Bacârea Vladimir Constantin, Văsieşiu Anca Meda, Săplăcan Irina, Fodor Raluca Śtefania, Grigorescu Bianca Liana
Department of Pathophysiology, University of Medicine, Pharmacy, Science and Technology 'George Emil Palade', Târgu Mureș, Mureș 540142, Romania.
Department of Simulation Applied in Medicine, University of Medicine, Pharmacy, Science and Technology 'George Emil Palade', Târgu Mureș, Mureș 540142, Romania.
Exp Ther Med. 2024 Mar 15;27(5):203. doi: 10.3892/etm.2024.12489. eCollection 2024 May.
The present study was a prospective observational single center study, enrolling 102 patients with sepsis, admitted in the Intensive Care Unit of the County Emergency Clinical Hospital in Târgu Mureș (Mureș, Romania). The main goal of the present study was to compare the changes of the following parameters on day 1 compared with day 5, in sepsis compared with septic shock, as well as in survivors compared with non-survivors: Cell blood count parameters, neutrophil-lymphocyte ratio, platelet-lymphocyte ratio and systemic inflammation index, C reactive protein (CRP), ferritin, procalcitonin (PCT), CD 3+ T cells, CD4+ T cells, CD8+ T cells, CD16+CD56+/CD3-NK cells and CD19+ B cells. The relationship between the subcategories of lymphocytes with the inflammatory markers was evaluated. The serum concentration of CRP and PCT was significantly lower on day 5 compared with day 1 and serum ferritin was significantly higher in patients with septic shock. The percentage of cytotoxic T lymphocytes was significantly decreased and the percentage of NK lymphocytes was significantly increased in patients who developed septic shock. The results indicated a negative significant correlation between the proportion of T lymphocytes and PCT concentration and a positive significant correlation between the proportion of B lymphocytes and PCT concentration.
本研究是一项前瞻性观察性单中心研究，纳入了102例脓毒症患者，这些患者均入住罗马尼亚特尔古穆列什县急诊临床医院重症监护病房。本研究的主要目的是比较脓毒症与脓毒性休克患者、幸存者与非幸存者在第1天和第5天下列参数的变化：血细胞计数参数、中性粒细胞与淋巴细胞比值、血小板与淋巴细胞比值和全身炎症指数、C反应蛋白（CRP）、铁蛋白、降钙素原（PCT）、CD3⁺ T细胞、CD4⁺ T细胞、CD8⁺ T细胞、CD16⁺CD56⁺/CD3⁻ 自然杀伤细胞（NK细胞）和CD19⁺ B细胞。评估了淋巴细胞亚类与炎症标志物之间的关系。与第1天相比，第5天CRP和PCT的血清浓度显著降低，脓毒性休克患者的血清铁蛋白显著升高。发生脓毒性休克的患者中，细胞毒性T淋巴细胞百分比显著降低，NK淋巴细胞百分比显著升高。结果表明，T淋巴细胞比例与PCT浓度呈显著负相关，B淋巴细胞比例与PCT浓度呈显著正相关。
