CHU Lille, Unité Fonctionnelle de Toxicologie, F-59037, Lille, France.
Ecole Nationale Vétérinaire, Agroalimentaire et de l'Alimentation Nantes-Atlantique - ONIRIS, Nantes, France.
Toxicon. 2022 Apr 30;210:39-43. doi: 10.1016/j.toxicon.2022.02.009. Epub 2022 Feb 18.
Analytical detection of Oenanthe crocata toxins in biological samples is challenging because of their instability, the lack of commercially available standards and the exceptionally low detection of these molecules using mass spectrometry. This work aims to report the used analytical methods that allowed identification of the main plant toxins in biological samples from an equid (an Arabian horse) fatality related to hemlock water dropwort (Oenanthe crocata Linnaeus) intake. Using both LC-DAD and LC-HRMS methods allowed identification (i) of oenanthotoxin in roots found on the site, root fragments found in the stomach, stomach content, kidney, and liver, and (ii) of the hydrogenated metabolite of oenanthotoxin (2,3-dihydro-oenanthotoxin) in roots found on the site, root fragments found in the stomach, stomach content, kidney, liver and spleen. Reported analytical data about Oenanthe crocata toxins can be useful for identification of the ingested plant and for supporting a poisoning diagnosis in such cases.
分析检测生物样本中的獐牙菜毒素具有挑战性，因为这些毒素不稳定，缺乏商业上可用的标准，并且使用质谱法对这些分子的检测灵敏度极低。本工作旨在报告所使用的分析方法，这些方法可鉴定与毒芹（Oenanthe crocata Linnaeus）摄入有关的马属动物（一匹阿拉伯马）死亡的生物样本中的主要植物毒素。使用 LC-DAD 和 LC-HRMS 方法，可鉴定在现场发现的獐牙菜根部、胃中发现的根部碎片、胃内容物、肾脏和肝脏中的獐牙菜毒素（oenanthotoxin），以及在现场发现的獐牙菜根部、胃中发现的根部碎片、胃内容物、肾脏、肝脏和脾脏中的獐牙菜毒素的氢化代谢物（2,3-二氢獐牙菜毒素）（2,3-dihydro-oenanthotoxin）。关于獐牙菜毒素的分析数据报告，可用于鉴定摄入的植物，并在这种情况下支持中毒诊断。
