State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, No. 2, Sipailou, Nanjing, 210096, PR China.
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, No. 2, Sipailou, Nanjing, 210096, PR China.
Talanta. 2022 May 15;242:122989. doi: 10.1016/j.talanta.2021.122989. Epub 2021 Oct 21.
Virus surveillance and discovery are crucial for virus prediction and outbreak preparedness. Virus samples are frequently bulky and complicated so that effective virus detection remain challenging. Herein, we develop an 3D electrostatic microfluidic platform to rapidly and label-free enrich viruses from bulky samples at low concentrations. The platform consists of double microchannels for streamlining large volume processing and electrodes for enriching viruses by electrostatic interaction. The trajectories of simulation show that particle is successfully enriched under different forces of electrostatic field and different sample flow rates. We demonstrate that the electrostatic microfluidic platform can increase the limit of detection in 100-fold higher based on real-time PCR quantified analysis. Our design thus provides a simple, rapid, label-free and high-throughput viruses concentration platform and would thus have significant utility for various viral detection.
病毒监测和发现对于病毒预测和爆发准备至关重要。病毒样本通常体积庞大且复杂，因此有效的病毒检测仍然具有挑战性。在此，我们开发了一种 3D 静电微流控平台，可从低浓度的大容量样本中快速且无需标记即可富集病毒。该平台由双微通道组成，用于简化大容量处理，以及由静电相互作用富集病毒的电极。模拟轨迹表明，在不同的静电场力和不同的样品流速下，颗粒成功地得到了富集。我们证明，基于实时 PCR 定量分析，静电微流控平台可以将检测限提高 100 倍。因此，我们的设计为各种病毒检测提供了一种简单、快速、无需标记和高通量的病毒浓缩平台，具有重要的应用价值。
