Department of Mechanical Engineering, Yonsei University, Seoul 03722, Republic of Korea.
Korea Electronics Technology Institute (KETI), Seongnam-si, Gyeonggi-do 13509, Republic of Korea.
J Hazard Mater. 2024 Mar 5;465:133249. doi: 10.1016/j.jhazmat.2023.133249. Epub 2023 Dec 14.
The severe acute respiratory syndrome (SARS-CoV-2) outbreak triggered global concern and emphasized the importance of virus monitoring. During a seasonal influenza A outbreak, relatively low concentrations of 10-10 viral genome copies are available per 1 m of air, which makes detection and monitoring very challenging because the limit of detection of most polymerase chain reaction (PCR) devices is approximately 10 viral genome copies/mL. In response to the urgent need for the rapid detection of airborne coronaviruses and influenza viruses, an electrostatic aerosol-to-hydrosol (ATH) sampler was combined with a concanavalin A (ConA)-coated high-throughput microfluidic chip. The samples were then used for PCR detection. The results revealed that the enrichment capacity of the ATH sampler was 30,000-fold for both HCoV-229E and H1N1 influenza virus, whereas the enrichment capacities provided by the ConA-coated microfluidic chip were 8-fold and 16-fold for HCoV-229E and H1N1 virus, respectively. Thus, the total enrichment capacities of our combined ATH sampler and ConA-coated microfluidic chip were 2.4 × 10-fold and 4.8 × 10-fold for HCoV-229E and H1N1 virus, respectively. This methodology significantly improves PCR detection by providing a higher concentration of viable samples.
严重急性呼吸系统综合征（SARS-CoV-2）的爆发引起了全球关注，并强调了病毒监测的重要性。在季节性甲型流感爆发期间，空气中每立方米的病毒基因组拷贝数相对较低，约为 10-10 拷贝，这使得检测和监测变得极具挑战性，因为大多数聚合酶链反应（PCR）设备的检测极限约为 10 拷贝/mL。为了应对快速检测空气传播冠状病毒和流感病毒的迫切需求，静电气溶胶到水溶胶（ATH）采样器与刀豆球蛋白 A（ConA）涂层高通量微流控芯片相结合。然后使用这些样本进行 PCR 检测。结果表明，ATH 采样器对 HCoV-229E 和 H1N1 流感病毒的富集能力分别为 30,000 倍，而 ConA 涂层微流控芯片对 HCoV-229E 和 H1N1 病毒的富集能力分别为 8 倍和 16 倍。因此，ATH 采样器和 ConA 涂层微流控芯片的总富集能力分别为 HCoV-229E 和 H1N1 病毒的 2.4×10 倍和 4.8×10 倍。这种方法通过提供更高浓度的有活力的样本，显著提高了 PCR 检测的灵敏度。
