Zhang Anlan, Chang Yuqing, Li Wen, Zhang Yuanbao, Wang Yuqian, Xie Haohan, Zuo Tao, Zhang Yu, Xi Jiyu, Wu Xin, Wei Zewen, Chen Rui
School of Medical Technology, Beijing Institute of Technology, Beijing 100081, China.
Beijing Key Laboratory of Occupational Safety and Health, Institute of Urban Safety and Environmental Science, Beijing Academy of Science and Technology, Beijing 100054, China.
Biosensors (Basel). 2025 Jul 23;15(8):475. doi: 10.3390/bios15080475.
Detecting airborne viruses using an integrated aerosol sampling detection device is of great significance in epidemic prevention and control. Most of the applicable aerosol samplers have a flow rate of less than 1000 L/min, which is insufficient for application in large public spaces. Recent research, on the other hand, has revealed the advantages of microfluidic chip-based LAMP-CRISPR in airborne virus detection; however, this promising detection method has yet to be integrated with an aerosol sampler. Herein, we present an aerosol sampling and microfluidic chip-based detection (ASMD) device that couples a high-flow-rate aerosol sampling (HFAS) system with a microfluidic LAMP-CRISPR detection (MLCD) chip for surveilling airborne viruses, as represented by SARS-CoV-2. The HFAS system achieved a 6912 L/min flow rate while retaining a satisfactory collection efficiency, and achieved an enrichment ratio of 1.93 × 10 that facilitated subsequent detection by the MLCD chip. The MLCD chip integrates the whole LAMP-CRISPR procedure into a single chip and is compatible with the HFAS system. Environmental detection experiments show the feasibility of the ASMD device for aerosol sampling and detection. Our ASMD device is a promising tool for large space aerosol detection for airborne virus surveillance.
使用集成式气溶胶采样检测装置检测空气传播病毒在疫情防控中具有重要意义。大多数适用的气溶胶采样器流速小于1000 L/分钟，这不足以应用于大型公共场所。另一方面，最近的研究揭示了基于微流控芯片的LAMP-CRISPR在空气传播病毒检测中的优势；然而，这种有前景的检测方法尚未与气溶胶采样器集成。在此，我们展示了一种基于气溶胶采样和微流控芯片的检测（ASMD）装置，该装置将高流速气溶胶采样（HFAS）系统与微流控LAMP-CRISPR检测（MLCD）芯片相结合，用于监测以SARS-CoV-2为代表的空气传播病毒。HFAS系统实现了6912 L/分钟的流速，同时保持了令人满意的收集效率，并实现了1.93×10的富集率，便于随后由MLCD芯片进行检测。MLCD芯片将整个LAMP-CRISPR程序集成到单个芯片中，并与HFAS系统兼容。环境检测实验表明了ASMD装置进行气溶胶采样和检测的可行性。我们的ASMD装置是用于大型空间气溶胶检测以监测空气传播病毒的一种有前景的工具。
