Osley M A, Gould J, Kim S, Kane M Y, Hereford L
Cell. 1986 May 23;45(4):537-44. doi: 10.1016/0092-8674(86)90285-0.
Sequences between a pair of divergently transcribed histone genes in Saccharomyces cerevisiae are able to confer periodic transcription during the cell cycle. This conclusion contrasts to our previous hypothesis that an ars (autonomously replicating sequence) 3' to this locus is a transcription timer for yeast histone genes. The promoter sequences required for periodic expression have been localized by deletion analysis, and isolated elements have been analyzed by insertion into a heterologous promoter. Two cell-cycle-specific promoter functions have been identified. One function activates transcription in a cell-cycle-dependent manner. The other periodically represses transcription. Negative regulation may be the predominant form of cell-cycle control, because removal of the repressing function results in constitutive expression of the histone genes.
酿酒酵母中一对反向转录的组蛋白基因之间的序列能够在细胞周期中赋予周期性转录。这一结论与我们之前的假设相反，即该位点3'端的一个自主复制序列（ars）是酵母组蛋白基因的转录定时器。通过缺失分析定位了周期性表达所需的启动子序列，并通过插入异源启动子对分离的元件进行了分析。已经鉴定出两种细胞周期特异性启动子功能。一种功能以细胞周期依赖性方式激活转录。另一种功能周期性地抑制转录。负调控可能是细胞周期控制的主要形式，因为去除抑制功能会导致组蛋白基因的组成型表达。
