el-Zaatari F A, Reiss E, Yakrus M A, Bragg S L, Kaufman L
Diagn Immunol. 1986;4(2):97-106.
Sera from rabbits immunized with culture filtrates and homogenates of Nocardia asteroides B1042 gave at least eight precipitin bands by immunoelectrophoresis. At least 20 proteins with isoelectric points (pls) in the pH 4 to 5.4 range were observed in isoelectric focusing patterns. The enzyme-linked immunoelectro-transfer blot (EITB) assay showed that several of the isofocused proteins reacted with rabbit antisera and with sera from nocardiosis and tuberculosis patients. Antibodies against three proteins with pls of 4, 4.43, and 4.68 (antigenic factors 1,6,8) were present in nocardiosis patients' sera. The proteins were excised from isofocused gels, and IgG monoclonal antibodies (MAbs) were produced by the hybridoma method. MAbs against factors 1 and 6 did not crossreact with cytoplasmic antigens of Mycobacterium chelonae, M. intracellulare serotypes 4B and 8A, M. fortuitum, M. gordonae, or M. kansasii in the EITB method. Factor 8 (MAb) crossreacted with antigens of M. intracellulare and M. fortuitum.
用星形诺卡菌B1042的培养滤液和匀浆免疫的兔血清通过免疫电泳至少产生八条沉淀带。在等电聚焦图谱中观察到至少20种等电点（pI）在pH 4至5.4范围内的蛋白质。酶联免疫电转移印迹（EITB）分析表明，几种等聚焦蛋白质与兔抗血清以及诺卡菌病和结核病患者的血清发生反应。诺卡菌病患者血清中存在针对三种等电点分别为4、4.43和4.68的蛋白质（抗原因子1、6、8）的抗体。从等聚焦凝胶中切下这些蛋白质，并用杂交瘤方法制备IgG单克隆抗体（MAb）。在EITB方法中，针对因子1和6的单克隆抗体不与龟分枝杆菌、细胞内分枝杆菌4B和8A血清型、偶然分枝杆菌、戈登分枝杆菌或堪萨斯分枝杆菌的细胞质抗原发生交叉反应。因子8（单克隆抗体）与细胞内分枝杆菌和偶然分枝杆菌的抗原发生交叉反应。
