Angeles A M, Sugar A M
J Infect Dis. 1987 Feb;155(2):292-6. doi: 10.1093/infdis/155.2.292.
Diagnosis of nocardiosis is often delayed because of the frequently nonspecific nature of the illness and because of the long incubation period required to isolate the organism in the microbiology laboratory. Past experience with serological methods for diagnosing nocardiosis have met with little success, primarily because there was no suitable antigen. Using a previously identified Nocardia asteroides-specific protein, we developed an enzyme immunoassay for the rapid diagnosis of nocardiosis. We evaluated sera from 22 patients with nocardiosis, 83 hospitalized controls, and 21 patients with tuberculosis. Twenty (91%) of the sera from patients with nocardiosis had titers of antibody greater than or equal to 1:256, whereas all of the sera from controls and from patients with tuberculosis had titers less than 1:256. This assay represents a highly sensitive and specific serodiagnostic tool for evaluating patients with possible nocardiosis.
诺卡菌病的诊断常常延迟，这是因为该病症状通常不具有特异性，还因为在微生物实验室分离出该病原体需要很长的潜伏期。过去采用血清学方法诊断诺卡菌病收效甚微，主要原因是没有合适的抗原。利用先前鉴定出的星型诺卡菌特异性蛋白，我们开发了一种酶免疫测定法用于快速诊断诺卡菌病。我们评估了22例诺卡菌病患者、83例住院对照者以及21例结核病患者的血清。诺卡菌病患者的血清中有20份（91%）抗体滴度大于或等于1:256，而对照者和结核病患者的所有血清滴度均低于1:256。该测定法是用于评估可能患有诺卡菌病患者的一种高度灵敏且特异的血清诊断工具。
