College of Food Science, South China Agricultural University, Guangzhou, Guangdong, 510630, P. R. China.
College of Engineering, South China Agricultural University, Guangzhou, 510630, P. R. China.
Food Funct. 2022 Mar 7;13(5):2952-2965. doi: 10.1039/d1fo03694g.
Green tea polyphenols (GTP) play an important role in shaping the gut microbiome, comprising a range of densely colonizing microorganisms, including bacteriophages. Previous studies focused on the effect of GTP on the bacteria in the gut microbiota. However, little is known about the role of GTP in the bacteriophage composition of healthy intestines. In this study, SPF male C57BL/6J mice were divided into a polyphenol-free diet group and a tea polyphenol diet group where drinking water was supplemented with 0.1% GTP for 28 days. The ultra-deep metagenomic sequencing of virus-like particle preparations and bacterial 16S rRNA sequencing were performed on mouse stool samples. Changes in the gut bacteriome, bacteriophages, and bacterial-bacteriophage correlations were then compared between the groups. The results revealed an abundance of Firmicutes, a significant decrease in Bacteroidetes, and a significant increase in the ratio of F/B after GTP exposure. The GTP altered the abundance (relative abundance > 1.00%) of (regulation rate of 89.78% and the abundance up-regulated by 0.89%) and (regulation rate of 99.70% and the abundance down-regulated by 1.77%). The abundance of (regulation rate of 60.17%) increased by 24.38% following GTP treatment. The GTP also altered the abundance of phage (regulation rate of 98.64% and the abundance up-regulated by 3.16%) and that of (regulation rate of 99.99% and the abundance down-regulated by 5.44%). It significantly increased the intestine's lytic phages and reduced the temperate phages by 29.22%. The dominant microorganisms (relative abundance >1.00%) of and had a significantly negative relationship with the phage and a significantly positive relationship with the prophage. Exposure to GTP positively promoted changes in the gut bacteriophage community and interaction network in the microbial community of the SPF mice. These findings highlight the importance of "profitable" bacteriophage-bacteria relationships and reveal a potential mechanism of GTP towards the regulation of intestinal flora intestinal phage communities.
绿茶多酚（GTP）在塑造肠道微生物群方面发挥着重要作用，其中包括一系列密集定植的微生物，包括噬菌体。先前的研究集中在 GTP 对肠道微生物群中细菌的影响上。然而，对于 GTP 在健康肠道噬菌体组成中的作用知之甚少。在这项研究中，将 SPF 雄性 C57BL/6J 小鼠分为无多酚饮食组和茶多酚饮食组，其中饮用水中补充 0.1%的 GTP 28 天。对小鼠粪便样本中的病毒样颗粒制剂和细菌 16S rRNA 测序进行超深度宏基因组测序。然后比较两组之间肠道细菌群、噬菌体和细菌噬菌体相关性的变化。结果表明，GTP 暴露后厚壁菌门的丰度增加，拟杆菌门的丰度显著减少，F/B 比值显著增加。GTP 改变了 的丰度（相对丰度>1.00%）和 （调控率 89.78%，丰度上调 0.89%）的丰度（调控率 99.70%，丰度下调 1.77%）。GTP 处理后 （调控率 60.17%，丰度上调 24.38%）的丰度增加。GTP 还改变了噬菌体（调控率 98.64%，丰度上调 3.16%）和噬菌体（调控率 99.99%，丰度下调 5.44%）的丰度。它显著增加了肠道的裂解噬菌体，并将温和噬菌体减少了 29.22%。和 的主要微生物（相对丰度>1.00%）与噬菌体呈显著负相关，与噬菌体呈显著正相关。GTP 暴露可促进 SPF 小鼠微生物群落中肠道噬菌体群落及其相互作用网络的变化。这些发现强调了“有利可图”的噬菌体-细菌关系的重要性，并揭示了 GTP 调节肠道菌群和肠道噬菌体群落的潜在机制。
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