Hammouda Hanan F, Farag Mohammad M, El Deftar Mervat M F, Abdel-Gabbar M, Mohamed Basant M
The Healthy Chemistry Department, Center Health Laboratory, Ministry of Health, Cairo, Egypt.
Glass Research Department, National Research Centre, 33 El Bohouth Str., Dokki, Giza, 12622, Egypt.
J Genet Eng Biotechnol. 2022 Feb 21;20(1):33. doi: 10.1186/s43141-022-00302-x.
Cerium-containing materials have wide applications in the biomedical field, because of the mimetic catalytic activities of cerium. The study aims to deeply estimate the biocompatibility of different scaffolds based on Ce-doped nanobioactive glass, collagen, and chitosan using the first passage of rabbit bone marrow mesenchymal stem cells (BM-MSCs) directed to osteogenic lineage by direct and indirect approach. One percentage of glass filler was used (30 wt. %) in the scaffold, while the percentage of CeO in the glass was ranged from 0 to 10 mol. %. Cytotoxicity was evaluated by monitoring of cell morphological changes and reduction in cell proliferation activity of BMMSCs maintained under osteogenic condition using proliferation assays, MTT assay for the direct contact of cells/scaffolds twice in a week, trypan blue and hemocytometer cell counting for indirect contact of cells/scaffolds extracts at day 7. Cell behaviors growth, morphology characteristics were monitored daily under a microscope and cell counting were conducted after 1 week of the incubation of the cells with the extracts of the four composite scaffolds in the osteogenic medium at the end of the week.
Showed that at 24 h after direct contact with composite scaffold, all scaffolds showed proliferation of cells > 50% and increased in cell density on day 7. The scaffold of the highest percentage of CeO in bioactive glass nanoparticles (sample CL/CH/C10) showed the lowest inhibition of cell proliferation (< 25%) at day 7. Moreover, the indirect cell viability test showed that all extracts from the four composite scaffolds did not demonstrate a toxic effect on the cells (inhibition value < 25%).
The addition of CeO to the glass composition improved the biocompatibility of the composite scaffold for the proliferation of rabbit bone marrow mesenchymal stem cells directed to osteogenic lineage.
含铈材料因其铈的模拟催化活性在生物医学领域有广泛应用。本研究旨在通过直接和间接方法，利用定向分化为成骨谱系的兔骨髓间充质干细胞（BM-MSCs）的首次传代，深入评估基于铈掺杂纳米生物活性玻璃、胶原蛋白和壳聚糖的不同支架的生物相容性。支架中使用了1%的玻璃填料（30 wt.%），而玻璃中CeO的百分比范围为0至10 mol.%。通过监测细胞形态变化以及使用增殖测定法监测在成骨条件下维持的BM-MSCs的细胞增殖活性降低来评估细胞毒性，每周两次通过MTT法检测细胞/支架直接接触的细胞毒性，在第7天通过台盼蓝和血细胞计数器细胞计数检测细胞/支架提取物间接接触的细胞毒性。每天在显微镜下监测细胞行为、生长和形态特征，并在细胞与四种复合支架提取物在成骨培养基中孵育1周后于周末进行细胞计数。
表明在与复合支架直接接触24小时后，所有支架在第7天均显示细胞增殖>50%且细胞密度增加。生物活性玻璃纳米颗粒中CeO百分比最高的支架（样品CL/CH/C10）在第7天显示出最低的细胞增殖抑制（<25%）。此外，间接细胞活力测试表明，四种复合支架的所有提取物均未对细胞表现出毒性作用（抑制值<25%）。
向玻璃组合物中添加CeO可改善复合支架对定向分化为成骨谱系的兔骨髓间充质干细胞增殖的生物相容性。
