Williams N E, Honts J E, Graeff R W
Exp Cell Res. 1986 Jun;164(2):295-310. doi: 10.1016/0014-4827(86)90030-3.
Two proteins from the Triton X-100-insoluble fraction of Tetrahymena pyriformis have been isolated and shown by immunological methods to be major components of a pervasive system of filaments localized within the oral apparatus. These proteins, OF-1 and OF-2, have apparent molecular weights (MWapp) in polyacrylamide gels of 87,000 and 80,000 D, respectively. Peptide maps obtained and the absence of immunological cross-reactivity suggest that these proteins are not closely related to each other. Indirect immunofluorescence studies on dividing cells have shown that the oral filament system forms late in the cell cycle. The filaments appeared first after the basal bodies in the oral primordium had organized into groups and the fission furrow had begun to form. Dedifferentiation of the oral filament system in the anterior (old) oral apparatus was also observed at this point in the cell cycle. Following this, the oral filament systems in both old and new oral apparatuses completed development synchronously. Proteins showing antigenic similarity to OF-1 were found in a number of other cell types. Tests with heterologous antisera failed to demonstrate a relationship between vertebrate cytoskeletal proteins and the oral filament proteins of Tetrahymena.
已从梨形四膜虫的Triton X - 100不溶性组分中分离出两种蛋白质，通过免疫学方法表明它们是位于口器内的广泛丝状系统的主要成分。这些蛋白质，即OF - 1和OF - 2，在聚丙烯酰胺凝胶中的表观分子量（MWapp）分别为87,000和80,000 D。所得的肽图以及缺乏免疫交叉反应表明这些蛋白质彼此之间没有密切关系。对分裂细胞的间接免疫荧光研究表明，口丝系统在细胞周期后期形成。在口原基中的基体组织成组且分裂沟开始形成后，丝首先出现。在细胞周期的这一阶段也观察到前（旧）口器中的口丝系统去分化。此后，旧口器和新口器中的口丝系统同步完成发育。在许多其他细胞类型中发现了与OF - 1具有抗原相似性的蛋白质。用异源抗血清进行的测试未能证明脊椎动物细胞骨架蛋白与四膜虫的口丝蛋白之间存在关系。
