Olivas-Quintero Sandra, Bernal-Reynaga Rodolfo, Lopez-Saucedo Catalina, Maldonado-Puga Samantha, Díaz-Camacho Sylvia Paz, Uribe-Carvajal Salvador, Delgado-Vargas Francisco, Estrada-Garcia Teresa
School of Chemical and Biological Sciences, Autonomous University of Sinaloa, Culiacan, Sinaloa, Mexico.
Molecular Biomedicine Department, CINVESTAV-IPN, Mexico City, Mexico.
J Infect Dev Ctries. 2022 Jan 31;16(1):147-156. doi: 10.3855/jidc.15125.
Diarrheagenic Escherichia coli pathotypes are important aetiological agents of diarrhoeal illness among children from less developed areas, worldwide. Diarrheagenic E. coli pathotypes strains are increasingly becoming drug resistant, thus effective and accessible therapeutic alternatives are required for their treatment; herbal extracts may be a potential alternative.
to evaluate Echeveria craigiana, E. kimnachii, and E. subrigida methanol extracts antibacterial effect on six diarrheagenic E. coli reference strains and on human colorectal adenocarcinoma cells viability and cytokine production.
Diarrheagenic E. coli pathotypes reference strains: typical enteropathogenic E2348/69, enterotoxigenic H10407, enterohaemorrhagic O157:H7/EDL933, enteroinvasive E11, diffusely adherent C18451-A, and enteroaggregative 042 E. coli. E craigiana, E. kimnachii, and E. subrigida leaves, collected at Sinaloa, Mexico, were freeze-dried and macerated in methanol solvent. Antibacterial activity was determined by a novel method developed in our laboratory, bacterial oxygen consumption by polarographic oxygen electrode technique and membrane integrity by two methods (live/dead and protein leakage assays). Colorectal adenocarcinoma cells viability by MTT assay and cytokine production using a Cytometric Bead Array kit.
Extracts concentrations of 100 μg/mL and 5-hour incubation, reduced more than 93% the growth of all diarrheagenic E. coli pathotypes tested strains and significantly decreased bacterial oxygen consumption, like bacteriostatic antibiotics. After 24-hour incubation methanol extracts had a differential antibacterial effect on each diarrheagenic E. coli pathotypes strain. Echeveria extracts did not have any effect on viability and cytokine production of colorectal adenocarcinoma cells.
Echeveria methanol extracts have a bacteriostatic effect on all diarrheagenic E. coli pathotypes strains, thus potentially they could be used as antibacterial agents on diarrheagenic E. coli pathotypes-contaminated products and on patients with diarrheagenic E. coli pathotypes infections.
致泻性大肠杆菌致病型是全球欠发达地区儿童腹泻疾病的重要病原体。致泻性大肠杆菌致病型菌株的耐药性日益增强，因此需要有效且可及的治疗替代方案；草药提取物可能是一种潜在的替代方案。
评估拟石莲花属克雷吉亚拟石莲花、金氏拟石莲花和硬叶拟石莲花甲醇提取物对六种致泻性大肠杆菌参考菌株以及对人结肠直肠腺癌细胞活力和细胞因子产生的抗菌作用。
致泻性大肠杆菌致病型参考菌株：典型致病性E2348/69、产肠毒素性H10407、肠出血性O157:H7/EDL933、侵袭性E11、弥漫性黏附性C18451-A和聚集性042大肠杆菌。在墨西哥锡那罗亚采集的拟石莲花属克雷吉亚拟石莲花、金氏拟石莲花和硬叶拟石莲花的叶子经冷冻干燥后，在甲醇溶剂中浸渍。抗菌活性通过我们实验室开发的一种新方法测定，采用极谱氧电极技术测定细菌耗氧量，并通过两种方法（死活细胞和蛋白质渗漏测定）测定膜完整性。通过MTT法测定结肠直肠腺癌细胞活力，并使用细胞计数珠阵列试剂盒测定细胞因子产生情况。
提取物浓度为100μg/mL且孵育5小时后，受试的所有致泻性大肠杆菌致病型菌株的生长减少了93%以上，细菌耗氧量显著降低，类似于抑菌抗生素。孵育24小时后，甲醇提取物对每种致泻性大肠杆菌致病型菌株具有不同的抗菌作用。拟石莲花属提取物对结肠直肠腺癌细胞的活力和细胞因子产生没有任何影响。
拟石莲花属甲醇提取物对所有致泻性大肠杆菌致病型菌株具有抑菌作用，因此它们有可能用作受致泻性大肠杆菌致病型污染产品以及致泻性大肠杆菌致病型感染患者的抗菌剂。
