细菌接合中高度重组的受体菌株。
Hyper-recombining recipient strains in bacterial conjugation.
作者信息
Feinstein S I, Low K B
出版信息
Genetics. 1986 May;113(1):13-33. doi: 10.1093/genetics/113.1.13.
Abstract
Using a direct enrichment and screening procedure, mutants of Escherichia coli have been isolated in which recombination frequencies for several intragenic Hfr X F- crosses are significantly higher (twofold to sixfold) than in the parental strains. These hyper-recombination mutations comprised five new mutS- and one new mutL- allele. Together with other known mut- alleles, they were analyzed for effects on intragenic recombination using several types of crosses. Hyper-recombination was found for mutS-, mutL-, mutH (= mutR)- and mutU (= uvrD)-, with the largest effects seen for certain alleles of uvrD; these resulted in over 20-fold excesses in recombinant production for Hfr X F- crosses and F'-chromosome homogenotization. Spontaneous mutator ability was not always correlated with degree of hyper-recombination.
摘要
通过直接富集和筛选程序，已分离出大肠杆菌突变体，其中几个基因内Hfr×F-杂交的重组频率比亲本菌株显著更高（两倍至六倍）。这些高重组突变包括五个新的mutS等位基因和一个新的mutL等位基因。与其他已知的mut等位基因一起，使用几种类型的杂交分析它们对基因内重组的影响。发现mutS-、mutL-、mutH（=mutR）-和mutU（=uvrD）-存在高重组现象，uvrD的某些等位基因影响最大；这些导致Hfr×F-杂交和F'-染色体同基因化的重组产生过量20倍以上。自发诱变能力并不总是与高重组程度相关。
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