Cizmecioglu Ahmet, Emsen Ayca, Sumer Sua, Ergun Dilek, Akay Cizmecioglu Hilal, Turk Dagi Hatice, Artac Hasibe
Department of Internal Medicine, Selcuk University Faculty of Medicine, Konya, Turkey.
Department of Pediatric Allergy and Immunology, Selcuk University Faculty of Medicine, Konya, Turkey.
Viral Immunol. 2022 Apr;35(3):273-282. doi: 10.1089/vim.2021.0196. Epub 2022 Feb 22.
Monocytes are one of the principal immune defense cells that encounter infectious agents. However, an essential role of monocytes has been shown in the spread of viruses throughout the human body. Considering this dilemma, this study aimed to evaluate monocyte subsets and Human Leukocyte Antigen-DR isotype () expressions in clinical coronavirus disease 2019 (COVID-19) cases. This prospective, multicenter, case-control study was conducted with COVID-19 patients and healthy controls. The patient group was divided into two subgroups according to disease severity (severe and non-severe). Three monocyte subsets (classical, CL; intermediate, INT; non-classical, NC) were analyzed with flow cytometry upon the patients' hospital admission. A total of 42 patients with COVID-19 and 30 controls participated in this study. The patients' conditions were either severe ( = 23) or non-severe ( = 19). All patients' monocyte and HLA-DR expressions were decreased compared with the controls ( < 0.05). Per disease severity, all monocyte subsets were not significant with disease severity; however, the HLA-DR expressions of CL monocytes ( = 0.002) and INT monocytes ( = 0.025) were more decreased in the severe patient group. In patients with various clinical features, NC monocytes were more affected. Based on these results, NC monocytes were more decreased in acute COVID-19 cases, though related various clinics decreased all monocyte subsets in these patients. Decreased monocyte HLA expressions may be a sign of immune suppression in severe patients, even when the percentage of monocyte levels has not decreased yet.
单核细胞是接触感染因子的主要免疫防御细胞之一。然而，单核细胞在病毒在人体全身传播中所起的重要作用已得到证实。考虑到这一困境，本研究旨在评估2019冠状病毒病（COVID-19）临床病例中的单核细胞亚群和人类白细胞抗原-DR同种型（）表达。本前瞻性、多中心、病例对照研究纳入了COVID-19患者和健康对照。患者组根据疾病严重程度分为两个亚组（重症和非重症）。患者入院时采用流式细胞术分析三个单核细胞亚群（经典型，CL；中间型，INT；非经典型，NC）。共有42例COVID-19患者和30名对照参与了本研究。患者病情为重症（n = 23）或非重症（n = 19）。与对照组相比，所有患者的单核细胞和HLA-DR表达均降低（P < 0.05）。按疾病严重程度划分，所有单核细胞亚群与疾病严重程度无显著相关性；然而，重症患者组中CL单核细胞（P = 0.002）和INT单核细胞（P = 0.025）的HLA-DR表达下降更为明显。在具有各种临床特征的患者中，NC单核细胞受影响更大。基于这些结果，急性COVID-19病例中NC单核细胞减少更为明显，尽管相关的各种临床情况使这些患者的所有单核细胞亚群均减少。单核细胞HLA表达降低可能是重症患者免疫抑制 的一个迹象，即使单核细胞水平的百分比尚未下降。
