Glycidyl methacrylate copolymers as suitable supports for immobilizing antigens and antibodies.

Glycidyl methacrylate copolymers modified with 1,6-diaminohexane were activated with glutaraldehyde. Such activated supports were directly bound to the chosen antisera. Prepared immunosorbents contained ca. 40 mg of bound protein and were able to purify 2-5 mg of antigen (alkaline proteinases Esperase and Maxatase) per 1 g of solid support. Chaotropic agent (2.5 M ammonium thiocyanate in phosphate or Tris buffer) enabled antigen to be released quickly, the properties of the immunosorbent remaining unchanged.