Zhang Xue, Yang Jian, Liu Zhaosheng, Huang Yanping, Akber Aisa Haji
State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Key Laboratory of Plant Resources and Chemistry in Arid Regions, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, People's Republic of China; University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Key Laboratory of Plant Resources and Chemistry in Arid Regions, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, People's Republic of China; Xinjiang Medical University, Urumqi 830011, Xinjiang, People's Republic of China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2022 Mar 15;1193:123172. doi: 10.1016/j.jchromb.2022.123172. Epub 2022 Feb 12.
This paper reported a feasible method to prepare molecularly imprinted polymer (MIPs) using 4-vinylpyridine (4-VP) and Allyl-β-cyclodextrin (β-CD) as binary functional monomer in the presence of polystyrene (PS). This is the first time that a surrounding of macromolecular crowding was established to improve the imprinting effect of cyclodextrins as monomer in organic solvents. The morphological and characteristics of the polymers with macromolecular crowding reagents were investigated by scanning electron microscope (SEM), fourier-transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TGA) and nitrogen adsorption experiments. The MIPs were synthesized with 4-VP and β-CD as binary functional monomers, a series of experiments were conducted to compare with the control groups. Furthermore, the selectivity of MIP for analogues experiment showed that the β-CD/4-VP MIP has higher specific recognition for arctiin than β-CD/4-VP NIP. A purification method by β-CD/4-VP MIPs coupled with macromolecular crowding reagents was developed for extraction arctiin from Arctium lappa L. In the MIP-SPE process, the optimal washing and eluting reagents are methanol/water (5:5) and methanol/acid (9:1), respectively. When using the β-CD/4-VP MIPs as SPE absorbent, the mean recoveries for arctiin were 87% with purity of 95%. All the results indicate that this synthetic method using 4-VP and β-CD as binary functional monomers in the presence of PS is a promising method for the preparation of selective adsorbents for arctiin analysis in Arctium lappa L.
本文报道了一种可行的方法，即在聚苯乙烯（PS）存在下，使用4-乙烯基吡啶（4-VP）和烯丙基-β-环糊精（β-CD）作为二元功能单体来制备分子印迹聚合物（MIPs）。这是首次建立大分子拥挤环境以提高环糊精作为有机溶剂中单体的印迹效果。通过扫描电子显微镜（SEM）、傅里叶变换红外光谱（FT-IR）、热重分析（TGA）和氮吸附实验研究了含有大分子拥挤试剂的聚合物的形态和特性。以4-VP和β-CD作为二元功能单体合成了MIPs，并进行了一系列实验与对照组进行比较。此外，MIP对类似物的选择性实验表明，β-CD/4-VP MIP对牛蒡子苷的特异性识别高于β-CD/4-VP非印迹聚合物（NIP）。开发了一种结合大分子拥挤试剂的β-CD/4-VP MIPs纯化方法，用于从牛蒡中提取牛蒡子苷。在MIP-固相萃取（SPE）过程中，最佳洗涤和洗脱试剂分别是甲醇/水（5:5）和甲醇/酸（9:1）。当使用β-CD/4-VP MIPs作为SPE吸附剂时，牛蒡子苷的平均回收率为87%，纯度为95%。所有结果表明，这种在PS存在下使用4-VP和β-CD作为二元功能单体的合成方法是一种制备用于牛蒡中牛蒡子苷分析的选择性吸附剂的有前景的方法。
