Li Long, Li Lanfu, Cheng Gege, Wei Sentao, Wang Yaohui, Huang Qin, Wu Wei, Liu Xiuyu, Chen Guoning
School of Chemistry and Chemical Engineering, Guangxi Minzu University, Nanning 530006, China.
Key Laboratory of Chemistry and Engineering of Forest Products, State Ethnic Affairs Commission, Nanning 530006, China.
Polymers (Basel). 2022 Jul 6;14(14):2771. doi: 10.3390/polym14142771.
Chrysin is a natural bioactive molecule with various groups, and it has been a challenge to separate and enrich chrysin from natural products. Molecularly imprinted polymers have been widely used in the extraction of natural products, but the number and type of functional monomers limits the separation effect. The synergistic action of multiple functional monomers can improve the separation effect. In this paper, molecularly imprinted polymers (Bi-MIPs) were prepared using methacrylic acid and acrylamide as binary functional monomers for the separation and enrichment of chrysin. The Bi-MIPs were characterized using thermogravimetric analyzer (TGA), Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscope (SEM). The performances of Bi-MIPs were assessed, which included adsorption isotherms, selective recognition and adsorption kinetics. The experimental results show that Bi-MIPs are shaped as a uniform sphere with an abundant pocket structure on its surface. The adsorption of chrysin on the Bi-MIPs followed a pseudo-second-order and adapted Langmuir-Freundlich isotherm models. The adsorption performance of the Bi-MIPs was determined at different temperatures, and the Bi-MIPs showed excellent adsorption performance at 30 °C. The initial decomposition temperature of the Bi-MIPs was 220 °C. After five times of adsorption and desorption, the adsorption performance of the Bi-MIPs decreased by only 7%. In contrast with single functional monomer molecularly imprinted polymers (Si-MIPs), the Bi-MIPs showed excellent specificity, with an imprinting factor of 1.54. The Bi-MIPs are promising materials in the separation and enrichment of chrysin for their high adsorption capacity, low cost and being environmentally friendly.
白杨素是一种具有多种基团的天然生物活性分子，从天然产物中分离和富集白杨素一直是一项挑战。分子印迹聚合物已广泛应用于天然产物的提取，但功能单体的数量和类型限制了分离效果。多种功能单体的协同作用可以提高分离效果。本文以甲基丙烯酸和丙烯酰胺为二元功能单体制备了分子印迹聚合物（Bi-MIPs），用于白杨素的分离和富集。采用热重分析仪（TGA）、傅里叶变换红外光谱仪（FT-IR）和扫描电子显微镜（SEM）对Bi-MIPs进行了表征。评估了Bi-MIPs的性能，包括吸附等温线、选择性识别和吸附动力学。实验结果表明，Bi-MIPs呈均匀球形，表面具有丰富的口袋结构。白杨素在Bi-MIPs上的吸附遵循准二级动力学，并符合Langmuir-Freundlich等温线模型。在不同温度下测定了Bi-MIPs的吸附性能，Bi-MIPs在30℃时表现出优异的吸附性能。Bi-MIPs的初始分解温度为220℃。经过五次吸附和解吸后，Bi-MIPs的吸附性能仅下降了7%。与单功能单体分子印迹聚合物（Si-MIPs）相比，Bi-MIPs表现出优异的特异性，印迹因子为1.54。Bi-MIPs因其高吸附容量、低成本和环境友好性，是白杨素分离和富集中很有前景的材料。
