Oie S, Koshiro A, Konishi H, Yoshii Z
J Clin Microbiol. 1986 Jun;23(6):1084-7. doi: 10.1128/jcm.23.6.1084-1087.1986.
The combined usage of fosfomycin (FOM) and 5-fluorouracil (5-FU) as selective agents for the isolation of leptospires from contaminated materials was investigated. Additive or synergistic antibacterial activity was apparent with the combination compared with each agent used separately. Of 54 bacterial strains tested, 52 were inhibited, while all 5 Leptospira strains tested were unaffected by the combined addition of FOM (400 micrograms/ml) and 5-FU (100 micrograms/ml) to Korthof medium. Furthermore, this combination successfully supported the selective growth of Leptospira interrogans serovar copenhageni in experimentally contaminated specimens. This FOM-5-FU combination is surmised to be useful for the selective isolation of leptospires from contaminated clinical, pathological, or environmental materials.
研究了磷霉素(FOM)和5-氟尿嘧啶(5-FU)联合用作从污染材料中分离钩端螺旋体的选择剂的情况。与单独使用每种药物相比,联合使用时具有明显的相加或协同抗菌活性。在测试的54株细菌菌株中,52株受到抑制,而在科托夫培养基中联合添加FOM(400微克/毫升)和5-FU(100微克/毫升)时,所有测试的5株钩端螺旋体菌株均未受影响。此外,这种组合成功地支持了哥本哈根问号钩端螺旋体在实验污染标本中的选择性生长。推测这种FOM-5-FU组合可用于从污染的临床、病理或环境材料中选择性分离钩端螺旋体。