Chakraborty Abhik, Kar Sujata, Mohapatra Purna Chandra, Banerjee Birendranath
KIIT, School of Biotechnology, KIIT University, Bhubaneswar, Odisha, India.
Kar Clinic and Hospitals Pvt Ltd, Bhubaneswar, Odisha, India.
J Hum Reprod Sci. 2021 Oct-Dec;14(4):422-430. doi: 10.4103/jhrs.jhrs_68_21. Epub 2021 Dec 31.
Recurrent pregnancy loss (RPL) is a common occurrence that affects up to 15% of couples in their reproductive years. In both males and females with RPL and infertility, chromosomal abnormalities play a significant impact.
The study was designed to examine the involvement of chromosomal anomalies and the frequency of certain chromosomal variants persistent among couples experiencing RPL.
This case-control study was conducted on 1000 couples from January 2015 to September 2020 in the state of Odisha, India, strictly adhering to principles of Helsinki Declaration (1975). The study was performed at the School of Biotechnology, KIIT University in collaboration with inDNA Life Sciences Private Limited.
A cohort of 1148 individuals with a history of RPL were selected for the study and they were screened with respect to fertile controls for the presence of any chromosomal anomaly using G-banding, nucleolar organizing region (NOR)-banding and fluorescence hybridisation wherever necessary.
The connection between distinct polymorphic variations and the occurrence of RPL was assessed using Fisher's exact test. Significant was defined as a ≤ 0.005.
One hundred and thirty-four individuals were found to harbor chromosomal anomalies. This study elucidates that along with balanced chromosomal translocations, the involvement of polymorphic variants also plays a significant role in cases of RPL.
The cumulative occurrence of chromosomal anomalies and variants across our cohort of 1148 individuals indicates that the chromosomal assessment of all couples experiencing RPL must be performed by all the clinicians. This study aids us in identifying chromosomal polymorphisms as major players of RPL in addition to novel chromosomal translocations.
复发性流产（RPL）是一种常见情况，影响高达15%处于生育年龄的夫妇。在患有RPL和不孕症的男性和女性中，染色体异常都有重大影响。
本研究旨在检查染色体异常的参与情况以及某些染色体变异在经历RPL的夫妇中持续存在的频率。
本病例对照研究于2015年1月至2020年9月在印度奥里萨邦对1000对夫妇进行，严格遵循《赫尔辛基宣言》（1975年）的原则。该研究在KIIT大学生物技术学院与inDNA生命科学私人有限公司合作进行。
选择1148名有RPL病史的个体组成队列进行研究，并在必要时使用G显带、核仁组织区（NOR）显带和荧光杂交对其进行筛选，以检测是否存在任何染色体异常，并与生育对照进行比较。
使用Fisher精确检验评估不同多态性变异与RPL发生之间的关联。显著性定义为P≤0.005。
发现134名个体存在染色体异常。本研究表明，除了平衡染色体易位外，多态性变异在RPL病例中也起着重要作用。
在我们1148名个体的队列中，染色体异常和变异的累积发生率表明，所有临床医生都必须对所有经历RPL的夫妇进行染色体评估。本研究有助于我们确定染色体多态性是RPL的主要因素，此外还有新的染色体易位。
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