Yang Yuhuan, Li Fengna, Guo Qiuping, Wang Wenlong, Zhang Lingyu, Yin Yunju, Gong Saiming, Han Mengmeng, Yin Yulong
College of Animal Science and Technology, Hunan Agricultural University, Changsha, China.
Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Key Laboratory of Agro-Ecological Processes in Subtropical Region, Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, China.
Front Vet Sci. 2022 Feb 7;8:828165. doi: 10.3389/fvets.2021.828165. eCollection 2021.
This study examined the effects of dietary leaf extract (ELE) supplements on carcass traits and lipid metabolism in growing-finishing pigs. A total of 144 crossbred (Duroc × Landrace × Yorkshire) piglets with an average initial weight of 10.11 ± 0.03 kg were randomly allotted to four treatment groups, each with six replicates and six piglets per replicate. Each group of pigs was fed a basal diet or a diet supplemented with increasing levels of ELE (0.1, 0.2, or 0.3%). The results showed that adding ELE had no negative effect on the growth performance of pigs. Dietary supplements of 0.1% ELE significantly increased carcass weight ( < 0.01), dressing percentage ( < 0.01), carcass length ( < 0.05), and eye muscle area ( < 0.05). Compared with the control group, a 0.2% ELE supplement significantly increased ( < 0.01) the levels of adiponectin, insulin-like growth factor 1, and hormone-sensitive lipase and lipoprotein lipase activity in the serum. Histological examination showed that ELE inhibited fat deposition in the backfat tissue. Lipid metabolism-related biochemical indices and mRNA expression levels were improved after supplementing diets with ELE. Moreover, all three levels of ELE dramatically upregulated ( < 0.05) the protein levels of p-AMPK-α and p-ACC. In summary, adding ELE to pig diets could improve the carcass traits of growing-finishing pigs and exert a lipid-lowering effect by activating the AMPK-ACC pathway and regulating mRNA expression levels related to lipid metabolism. Supplementing the diet with 0.1-0.2% ELE is the optimal range to reduce fat deposition in pig backfat tissue.
本研究考察了日粮添加树叶提取物（ELE）对生长育肥猪胴体性状和脂质代谢的影响。总共144头平均初始体重为10.11±0.03千克的杂交（杜洛克×长白×约克夏）仔猪被随机分配到四个处理组，每组六个重复，每个重复六头仔猪。每组猪饲喂基础日粮或添加不同水平ELE（0.1%、0.2%或0.3%）的日粮。结果表明，添加ELE对猪的生长性能没有负面影响。日粮添加0.1% ELE显著增加了胴体重（P<0.01）、屠宰率（P<0.01）、胴体长度（P<0.05）和眼肌面积（P<0.05）。与对照组相比，添加0.2% ELE显著提高了血清中脂联素、胰岛素样生长因子1、激素敏感性脂肪酶和脂蛋白脂肪酶的活性（P<0.01）。组织学检查表明，ELE抑制了背膘组织中的脂肪沉积。日粮添加ELE后，脂质代谢相关生化指标和mRNA表达水平得到改善。此外，所有三个ELE水平均显著上调了p-AMPK-α和p-ACC的蛋白水平（P<0.05）。综上所述，在猪日粮中添加ELE可以改善生长育肥猪的胴体性状，并通过激活AMPK-ACC途径和调节脂质代谢相关mRNA表达水平发挥降脂作用。日粮添加0.1-0.2% ELE是减少猪背膘组织脂肪沉积的最佳范围。
