钙成像和 BAPTA 负载在小鼠脑片的杏仁核星形胶质细胞中。
Calcium imaging and BAPTA loading of amygdala astrocytes in mouse brain slices.
机构信息
Centre National de la Recherche Scientifique and University of Strasbourg, Institute of Cellular and Integrative Neuroscience, INCI CNRS UPR3212, 8, Allée du Général Rouvillois, Strasbourg, 67000 France.
Department of Neuropeptide Research in Psychiatry, Central Institute of Mental Health, University of Heidelberg, Mannheim, 68159 Germany.
出版信息
STAR Protoc. 2022 Feb 8;3(1):101159. doi: 10.1016/j.xpro.2022.101159. eCollection 2022 Mar 18.
Astrocytes are glial cells that exhibit calcium signaling-mediated activity. Here, we present a protocol to monitor and manipulate astrocyte calcium activity from mouse amygdala slices. In the first part of this protocol, we describe the procedure of astrocyte calcium imaging. In the second part, we detail how to disrupt astrocyte calcium activity by patch-clamp-mediated loading of BAPTA. These two approaches are presented separately but they can also be used simultaneously to monitor the effects of disruption on an astrocyte network. For complete details on the use and execution of this protocol, please refer to Wahis et al. (2021).
星形胶质细胞是具有钙信号介导活性的神经胶质细胞。在这里，我们提供了一个从小鼠杏仁核切片中监测和操纵星形胶质细胞钙活性的方案。在本方案的第一部分，我们描述了星形胶质细胞钙成像的过程。在第二部分，我们详细介绍了如何通过膜片钳介导的 BAPTA 加载来破坏星形胶质细胞钙活性。这两种方法是分开介绍的，但也可以同时使用，以监测对星形胶质细胞网络的破坏效果。有关此方案使用和执行的完整详细信息，请参阅 Wahis 等人。(2021)。
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