• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

从伊豆-赤泽深海海水中分离得到的新菌株 BF1-13 产生的代谢产物可保护肠道上皮屏障免受过氧化氢诱导的功能障碍。

Metabolites Produced by a New Strain BF1-13 Isolated from Deep Seawater of Izu-Akazawa Protect the Intestinal Epithelial Barrier from the Dysfunction Induced by Hydrogen Peroxide.

机构信息

Applied Microbiology Lab, Course of Applied Marine Biosciences, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, Tokyo 108-8477, Japan.

DSW Laboratory of DHC Co., Ltd., Tokyo 106-0047, Japan.

出版信息

Mar Drugs. 2022 Jan 20;20(2):87. doi: 10.3390/md20020087.

DOI:10.3390/md20020087
PMID:35200617
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8878880/
Abstract

This study aimed to investigate the protective effect of the metabolites produced by a new strain BF1-13, isolated from deep seawater (DSW), on the intestinal epithelial barrier against the dysfunction induced by hydrogen peroxide (HO) and to elucidate the mechanism underlying the effect. Protective effect of the metabolites by strain BF1-13 on the barrier function of the intestinal epithelial model treated with HO was investigated by the transepithelial electrical resistance (TEER). The metabolites enhanced the Claudin-4 (CLDN-4) expression, including at the transcription level, indicated by immunofluorescence staining and quantitative RT-PCR. The metabolites also showed a suppression of aquaporin3 (AQP3) expression. Lactic acid (LA) produced by this strain of homofermentative lactic acid bacteria (LAB) had a similar enhancement on CLDN-4 expression. The metabolites of strain BF1-13 alleviated the dysfunction of intestinal epithelial barrier owing to its enhancement on the tight junctions (TJs) by LA, along with its suppression on AQP3-facilitating HO intracellular invasion into Caco-2 cells. This is the first report on the enhancement of TJs by LA produced by LAB.

摘要

本研究旨在探讨一种从深海（DSW）中分离的新菌株 BF1-13 产生的代谢产物对肠道上皮屏障的保护作用，以防止其免受过氧化氢（HO）引起的功能障碍，并阐明其作用机制。通过跨上皮电阻（TEER）研究了 BF1-13 代谢产物对 HO 处理的肠道上皮模型屏障功能的保护作用。代谢产物通过免疫荧光染色和定量 RT-PCR 增强了紧密连接蛋白 4（CLDN-4）的表达，包括转录水平。代谢产物还显示出对水通道蛋白 3（AQP3）表达的抑制。该同型发酵乳酸菌（LAB）产生的乳酸（LA）对 CLDN-4 表达也有类似的增强作用。BF1-13 菌株的代谢产物通过 LA 增强紧密连接（TJ），同时抑制 AQP3 促进 HO 细胞内入侵 Caco-2 细胞，从而减轻肠道上皮屏障功能障碍。这是 LAB 产生的 LA 增强 TJ 的首次报道。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a97/8878880/050f8e933e00/marinedrugs-20-00087-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a97/8878880/eeea51838e27/marinedrugs-20-00087-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a97/8878880/8ea0c4c3d8cf/marinedrugs-20-00087-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a97/8878880/489008e5af2e/marinedrugs-20-00087-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a97/8878880/245360f8b4f6/marinedrugs-20-00087-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a97/8878880/da6c4a31b2b9/marinedrugs-20-00087-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a97/8878880/d4119fb20c3e/marinedrugs-20-00087-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a97/8878880/c9a7a27dae4f/marinedrugs-20-00087-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a97/8878880/1171ed82a89c/marinedrugs-20-00087-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a97/8878880/00da25400887/marinedrugs-20-00087-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a97/8878880/12f500f3d731/marinedrugs-20-00087-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a97/8878880/050f8e933e00/marinedrugs-20-00087-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a97/8878880/eeea51838e27/marinedrugs-20-00087-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a97/8878880/8ea0c4c3d8cf/marinedrugs-20-00087-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a97/8878880/489008e5af2e/marinedrugs-20-00087-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a97/8878880/245360f8b4f6/marinedrugs-20-00087-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a97/8878880/da6c4a31b2b9/marinedrugs-20-00087-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a97/8878880/d4119fb20c3e/marinedrugs-20-00087-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a97/8878880/c9a7a27dae4f/marinedrugs-20-00087-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a97/8878880/1171ed82a89c/marinedrugs-20-00087-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a97/8878880/00da25400887/marinedrugs-20-00087-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a97/8878880/12f500f3d731/marinedrugs-20-00087-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a97/8878880/050f8e933e00/marinedrugs-20-00087-g011.jpg

相似文献

1
Metabolites Produced by a New Strain BF1-13 Isolated from Deep Seawater of Izu-Akazawa Protect the Intestinal Epithelial Barrier from the Dysfunction Induced by Hydrogen Peroxide.从伊豆-赤泽深海海水中分离得到的新菌株 BF1-13 产生的代谢产物可保护肠道上皮屏障免受过氧化氢诱导的功能障碍。
Mar Drugs. 2022 Jan 20;20(2):87. doi: 10.3390/md20020087.
2
Protective effects of Lactobacillus plantarum on epithelial barrier disruption caused by enterotoxigenic Escherichia coli in intestinal porcine epithelial cells.植物乳杆菌对产肠毒素大肠杆菌引起的猪肠道上皮细胞上皮屏障破坏的保护作用。
Vet Immunol Immunopathol. 2016 Apr;172:55-63. doi: 10.1016/j.vetimm.2016.03.005. Epub 2016 Mar 5.
3
Fucoidan enhances intestinal barrier function by upregulating the expression of claudin-1.岩藻聚糖通过上调 Claudin-1 的表达增强肠道屏障功能。
World J Gastroenterol. 2013 Sep 7;19(33):5500-7. doi: 10.3748/wjg.v19.i33.5500.
4
Lactobacillus plantarum MB452 enhances the function of the intestinal barrier by increasing the expression levels of genes involved in tight junction formation.植物乳杆菌 MB452 通过增加参与紧密连接形成的基因的表达水平来增强肠道屏障功能。
BMC Microbiol. 2010 Dec 9;10:316. doi: 10.1186/1471-2180-10-316.
5
Lactiplantibacillus plantarum E51 protects against Clostridioides difficile-induced damages on Caco-2 intestinal barrier functions.植物乳杆菌 E51 可预防艰难梭菌诱导的 Caco-2 肠屏障功能损伤。
Arch Microbiol. 2022 May 3;204(6):290. doi: 10.1007/s00203-022-02837-6.
6
L. Tubers (Tiger Nuts) Protect Epithelial Barrier Function in Caco-2 Cells Infected by Enteritidis and Promote Growth.L. Tubers (Tiger Nuts) 可保护 Caco-2 细胞中受 Enteritidis 感染的上皮屏障功能并促进其生长。
Nutrients. 2020 Dec 28;13(1):71. doi: 10.3390/nu13010071.
7
Conversion of notoginsenoside R1 to 3β,12β-dihydroxydammar-(E)-20(22),24-diene-6-O-β-D-xylopyranosyl-(1→2)-β-D-glucopyranoside by Lactiplantibacillus plantarum S165 enhanced protective effects of LPS-induced intestinal epithelial barrier injury in Caco-2 cells.植物乳杆菌 S165 转化三七皂苷 R1 为 3β,12β-二羟基达玛-(E)-20(22),24-二烯-6-O-β-D-吡喃木糖基-(1→2)-β-D-吡喃葡萄糖苷增强了其对 LPS 诱导的 Caco-2 细胞肠上皮屏障损伤的保护作用。
J Appl Microbiol. 2024 Jul 2;135(7). doi: 10.1093/jambio/lxae180.
8
Lactobacillus plantarum L9 but not Lactobacillus acidophilus LA reduces tumour necrosis factor induced bacterial translocation in Caco-2 cells.植物乳杆菌L9可减少肿瘤坏死因子诱导的Caco-2细胞中的细菌易位，而嗜酸乳杆菌LA则不能。
Benef Microbes. 2017 May 30;8(3):497-505. doi: 10.3920/BM2016.0019. Epub 2017 Apr 26.
9
Lactobacillus plantarum inhibits intestinal epithelial barrier dysfunction induced by unconjugated bilirubin.植物乳杆菌可抑制由未结合胆红素诱导的肠上皮屏障功能障碍。
Br J Nutr. 2010 Aug;104(3):390-401. doi: 10.1017/S0007114510000474. Epub 2010 Apr 23.
10
Exopolysaccharide from HY7714 Protects against Skin Aging through Skin-Gut Axis Communication.HY7714 胞外多糖通过皮肤-肠道轴通讯保护皮肤衰老。
Molecules. 2021 Mar 16;26(6):1651. doi: 10.3390/molecules26061651.

引用本文的文献

1
Postbiotic Potential of Newly Isolated Riboflavin-Overproducing Lactiplantibacillus plantarum Strains.新分离的高产核黄素植物乳杆菌菌株的后生元潜力
Probiotics Antimicrob Proteins. 2025 Apr 23. doi: 10.1007/s12602-025-10538-x.
2
Protection of Tight Junctional Complexes between hCMEC/D3 Cells by Deep-Sea Fibrinolytic Compound FGFC1.深海纤维蛋白溶解复合物 FGFC1 对 hCMEC/D3 细胞紧密连接复合体的保护作用。
Mar Drugs. 2024 Jul 26;22(8):341. doi: 10.3390/md22080341.
3
Critical Signaling Transduction Pathways and Intestinal Barrier: Implications for Pathophysiology and Therapeutics.

本文引用的文献

1
D-Lactic Acid as a Metabolite: Toxicology, Diagnosis, and Detection.D-乳酸作为一种代谢物：毒理学、诊断和检测。
Biomed Res Int. 2020 Jun 17;2020:3419034. doi: 10.1155/2020/3419034. eCollection 2020.
2
Aquaporin-3 in the epidermis: more than skin deep.水通道蛋白-3 在表皮中的作用：不止于皮肤表面。
Am J Physiol Cell Physiol. 2020 Jun 1;318(6):C1144-C1153. doi: 10.1152/ajpcell.00075.2020. Epub 2020 Apr 8.
3
Dining With Inflammatory Bowel Disease: A Review of the Literature on Diet in the Pathogenesis and Management of IBD.
关键信号转导通路与肠道屏障：对病理生理学和治疗学的影响
Pharmaceuticals (Basel). 2023 Aug 29;16(9):1216. doi: 10.3390/ph16091216.
炎性肠病患者的饮食：饮食在炎症性肠病发病机制和治疗中的作用的文献综述。
Inflamm Bowel Dis. 2020 Jan 6;26(2):181-191. doi: 10.1093/ibd/izz268.
4
Risk of colorectal cancer in inflammatory bowel diseases.炎症性肠病相关结直肠癌的发病风险。
Semin Cancer Biol. 2020 Aug;64:51-60. doi: 10.1016/j.semcancer.2019.05.001. Epub 2019 May 18.
5
Kefir micro-organisms: their role in grain assembly and health properties of fermented milk.克菲尔微生物：它们在谷物组装中的作用和发酵乳的健康特性。
J Appl Microbiol. 2019 Mar;126(3):686-700. doi: 10.1111/jam.14107. Epub 2018 Oct 18.
6
Human Intestinal Barrier Function in Health and Disease.健康与疾病状态下的人类肠道屏障功能
Clin Transl Gastroenterol. 2016 Oct 20;7(10):e196. doi: 10.1038/ctg.2016.54.
7
Tight junctions in inflammatory bowel diseases and inflammatory bowel disease associated colorectal cancer.炎症性肠病及炎症性肠病相关结直肠癌中的紧密连接
World J Gastroenterol. 2016 Mar 21;22(11):3117-26. doi: 10.3748/wjg.v22.i11.3117.
8
Role of the normal gut microbiota.正常肠道微生物群的作用。
World J Gastroenterol. 2015 Aug 7;21(29):8787-803. doi: 10.3748/wjg.v21.i29.8787.
9
TEER measurement techniques for in vitro barrier model systems.体外屏障模型系统的跨上皮电阻测量技术。
J Lab Autom. 2015 Apr;20(2):107-26. doi: 10.1177/2211068214561025. Epub 2015 Jan 13.
10
Epidermal barriers.表皮屏障
Cold Spring Harb Perspect Med. 2014 Apr 1;4(4):a018218. doi: 10.1101/cshperspect.a018218.