R&BD Center, Korea Yakult Co Ltd., 22, Giheungdanji-ro 24 beon-gil, Giheung-gu, Gyeonggi-do 17086, Korea.
Molecules. 2021 Mar 16;26(6):1651. doi: 10.3390/molecules26061651.
Skin aging occurs inevitably as a natural result of physiological changes over time. In particular, solar exposure of the skin accounts for up to 90% of skin damage. Numerous studies have examined the ability of dietary constituents to prevent skin aging, and recent research has emphasized the role of functional probiotics in intestinal function and skin aging. However, the mechanism of the interactions between aging and probiotics has not been elucidated yet. The aim of this study was to determine the role of exopolysaccharides (EPS) produced by lactic acid bacteria (LAB) identified as HY7714 in regulating tight junctions in intestinal epithelial cells and increasing moisture retention in human dermal fibroblasts cells. We observed that HY7714 EPS controlled intestinal tight junctions in Caco-2 cells by upregulating the genes encoding occludin-1 (OCL-1) and zonula occluden-1 (ZO-1). In addition, HY7714 EPS effectively improved UVB-induced cytotoxicity and hydration capacity in HS68 cells by downregulating production of metalloproteinases (MMPs) and reactive oxygen species (ROS). In summary, HY7714 EPS is an effective anti-aging molecule in skin and may have therapeutic potential against skin diseases and UVB-induced damage. Therefore, HY7714 EPS serves as a functional substance in skin-gut axis communication.
皮肤衰老不可避免地是随着时间的推移而发生的生理变化的自然结果。特别是，皮肤的阳光暴露占皮肤损伤的 90%。许多研究已经研究了饮食成分预防皮肤衰老的能力，最近的研究强调了功能性益生菌在肠道功能和皮肤衰老中的作用。然而，衰老和益生菌之间相互作用的机制尚未阐明。本研究旨在确定鉴定为 HY7714 的乳酸菌（LAB）产生的胞外多糖（EPS）在调节肠道上皮细胞中的紧密连接和增加人真皮成纤维细胞细胞的保水能力中的作用。我们观察到 HY7714 EPS 通过上调编码闭合蛋白-1（OCL-1）和封闭蛋白-1（ZO-1）的基因来控制 Caco-2 细胞中的肠道紧密连接。此外，HY7714 EPS 通过下调金属蛋白酶（MMPs）和活性氧（ROS）的产生，有效改善了 HS68 细胞中 UVB 诱导的细胞毒性和水合能力。总之，HY7714 EPS 是一种有效的皮肤抗衰老分子，可能具有治疗皮肤病和 UVB 诱导损伤的潜力。因此，HY7714 EPS 是皮肤-肠道轴通讯的功能性物质。
