海洋来源的氮代谢物对的异柠檬酸裂解酶的抑制作用。

Inhibitory Effects of Nitrogenous Metabolites from a Marine-Derived on Isocitrate Lyase of .

机构信息

Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea.

Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul 08826, Korea.

出版信息

Mar Drugs. 2022 Feb 13;20(2):138. doi: 10.3390/md20020138.

DOI:10.3390/md20020138

PMID:35200667

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8878140/

Abstract

Two nitrogenous metabolites, bacillimide () and bacillapyrrole (), were isolated from the culture broth of the marine-derived actinomycete . Based on the results of combined spectroscopic and chemical analyses, the structure of bacillimide () was determined to be a new cyclopenta[]pyrrole-1,3-dione bearing a methylsulfide group, while the previously reported bacillapyrrole () was fully characterized for the first time as a pyrrole-carboxamide bearing an alkyl sulfoxide side chain. Bacillimide () and bacillapyrrole () exerted moderate (IC = 44.24 μM) and weak (IC = 190.45 μM) inhibitory effects on isocitrate lyase, respectively. Based on the growth phenotype using -deletion mutants and expression analyses, we determined that bacillimide () inhibits the transcriptional level of in under C-carbon-utilizing conditions.

摘要

从海洋来源放线菌中分离到两种含氮代谢物，棒曲霉素（）和棒曲霉素（）。基于综合光谱和化学分析的结果，确定棒曲霉素（）的结构为一种新型的含有甲基硫醚基团的环戊[]吡咯-1,3-二酮，而先前报道的棒曲霉素（）则首次被全称为带有烷基磺酰氧基侧链的吡咯酰胺。棒曲霉素（）和棒曲霉素（）对异柠檬酸裂解酶分别表现出中等（IC = 44.24 μM）和较弱（IC = 190.45 μM）的抑制作用。基于 - 缺失突变体的生长表型和表达分析，我们确定棒曲霉素（）在 C 碳利用条件下抑制中的转录水平。