Virtual optical-resolution photoacoustic microscopy using the k-Wave method.

Deep learning has been widely used in image processing, quantitative analysis, and other applications in optical-resolution photoacoustic microscopy (OR-PAM). It requires a large amount of photoacoustic data for training and testing. However, due to the complex structure, high cost, slow imaging speed, and other factors of OR-PAM, it is difficult to obtain enough data required by deep learning, which limits the research of deep learning in OR-PAM to a certain extent. To solve this problem, a virtual OR-PAM based on k-Wave is proposed. The virtual photoacoustic microscopy mainly includes the setting of excitation light source and ultrasonic probe, scanning and signal processing, which can realize the common Gaussian-beam and Bessel-beam OR-PAMs. The system performance (lateral resolution, axial resolution, and depth of field) was tested by imaging a vertically tilted fiber, and the effectiveness and feasibility of the virtual simulation platform were verified by 3D imaging of the virtual vascular network. The ability to the generation of the dataset for deep learning was also verified. The construction of the virtual OR-PAM can promote the research of OR-PAM and the application of deep learning in OR-PAM.