Abdel Maksoud Mohamed K M, Ibrahim Azza A H, Nabil Taghreed Mohamed, Moawad Usama Kamal
Anatomy and Embryology Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt.
Cytology and Histology Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt.
Anat Histol Embryol. 2022 May;51(3):380-389. doi: 10.1111/ahe.12798. Epub 2022 Feb 24.
The current investigation aimed to clarify the histomorphological features, scanning electron microscopy (SEM) and histochemistry of the proventriculus in the adult hooded crow (Corvus cornix). Twenty-two adult birds of both sexes were collected from El-Fayoum and Beni-Suef governorates, Egypt; ten were used for anatomical investigation, two were scanned using SEM, and ten were subjected to routine histological technique. The obtained anatomical results revealed a small-sized spindle-shaped proventriculus obliquely located in the left ventral part of the body cavity with an average body weight, length and diameter of 1.689 ± 0.231 gm, 1.54 ± 0.383cm and 3.51 ± 0.416cm, respectively. The histological examination revealed a typical tubular organ. The mucosa exhibited several plicae and sulci that were lined with simple columnar cells. The lamina propria was composed of collagenous connective tissue infiltrated by lymphocytes, lymphatic aggregations and tubular glands. The submucosa consisted of collagenic fibres and compound tubuloalveolar glandular lobules lined with oxynticopeptic cells and enteroendocrine cells. The oxynticopeptic cells reacted negatively with PAS and Alcian Blue stains, whereas the ductal cells reacted positively with PAS only. Enteroendocrine cells were seen singly in the lamina epithelialis mucosae and were abundant in submucosal glands. The tunica musclaris was arranged in two layers covered by serosa. The proventricular glands' openings were noticed by SEM as raised tubes with regular circular outlines surrounded by anastomosed microscopic folds. The obtained histomorphological structure of the hooded crow's proventriculus revealed some shared morphological features with most species of birds and some variations that might be attributed to their diet and feeding behaviour.
当前的研究旨在阐明成年 hooded crow（Corvus cornix）前胃的组织形态学特征、扫描电子显微镜（SEM）观察结果及组织化学特征。从埃及法尤姆省和贝尼苏韦夫省收集了 22 只成年雌雄鸟；其中 10 只用于解剖学研究，2 只进行 SEM 扫描，10 只进行常规组织学技术处理。所获得的解剖学结果显示，前胃呈小型纺锤形，斜位于体腔左侧腹部分，平均体重、长度和直径分别为 1.689 ± 0.231 克、1.54 ± 0.383 厘米和 3.51 ± 0.416 厘米。组织学检查显示为典型的管状器官。黏膜呈现出几条皱襞和沟，内衬单层柱状细胞。固有层由胶原结缔组织组成，有淋巴细胞、淋巴聚集物和管状腺浸润。黏膜下层由胶原纤维和复合管泡状腺小叶组成，内衬壁细胞和肠内分泌细胞。壁细胞对 PAS 和阿尔辛蓝染色呈阴性反应，而导管细胞仅对 PAS 呈阳性反应。肠内分泌细胞单个见于黏膜上皮层，在黏膜下腺中丰富。肌层分为两层，被浆膜覆盖。SEM 观察到前胃腺开口为隆起的管子，有规则的圆形轮廓，周围有吻合的微小褶皱。所获得的 hooded crow 前胃的组织形态学结构显示，与大多数鸟类物种有一些共同的形态学特征，也有一些可能归因于其饮食和取食行为的差异。
