Szewczyk B, Bienkowska-Szewczyk K, Kozloff L M
Mol Gen Genet. 1986 Mar;202(3):363-7. doi: 10.1007/BF00333263.
The proteins synthesized in Escherichia coli B cells after infection with various T4 bacteriophage tail baseplate mutants were analysed by the immunoblotting method for the presence of the 15 Kilodalton lysozyme found in phage T4 particles. Using three different antisera: anti-phage, anti-baseplate and anti-15K lysozyme, it has been found that the 15K lysozyme is not present in lysates of bacteria infected with T4 gene 25 amber mutants. The 15K lysozyme was also found to be expressed in E. coli B cells transformed with a plasmid containing only a small portion of the T4 genome but which included T4 gene 25. These observations indicate that the 15K lysozyme is the gene 25 product.
用免疫印迹法分析了用各种T4噬菌体尾基板突变体感染大肠杆菌B细胞后合成的蛋白质，以检测噬菌体T4颗粒中存在的15千道尔顿溶菌酶。使用三种不同的抗血清：抗噬菌体、抗基板和抗15K溶菌酶，发现用T4基因25琥珀突变体感染的细菌裂解物中不存在15K溶菌酶。还发现15K溶菌酶在用仅含有T4基因组一小部分但包含T4基因25的质粒转化的大肠杆菌B细胞中表达。这些观察结果表明15K溶菌酶是基因25的产物。
