College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu Province, 225009, P. R. China.
Department of Pathology, Linyi People's Hospital, Linyi 276000, Shandong Province, China.
Poult Sci. 2022 Apr;101(4):101734. doi: 10.1016/j.psj.2022.101734. Epub 2022 Jan 17.
Red light (RL) can improve egg production in Jinghai Yellow hens. Circular RNAs (circRNAs) are novel, non-coding RNAs, but the molecular mechanism underlying circRNA function during follicular development in hens under monochromatic light has not been established. Herein, we compared expression profiles of granulosa cells (GCs) from small yellow follicles (SYFs) from hens under RL and white light (WL). A total of 2,468 circRNAs were identified, of which 22 were differentially expressed (DE) in the RL and WL groups. DE circRNA host genes were enriched in ovarian steroidogenesis, and MAPK and PI3K-Akt signaling pathways. Furthermore, DE circRNA_0320 and circRNA_0185 interacted with miR-143-3p, which targets the follicle-stimulating hormone receptor and is essential for GC differentiation and follicle development. These findings will facilitate further analysis of the molecular mechanism leading to GC development in hens raised under monochromatic light, which could lead to increased egg production.
红光（RL）可以提高京海黄鸡的产蛋量。环状 RNA（circRNA）是一种新型的非编码 RNA，但在单色光照下母鸡卵泡发育过程中 circRNA 功能的分子机制尚不清楚。在此，我们比较了 RL 和白光（WL）下小黄色卵泡（SYF）母鸡颗粒细胞（GC）的表达谱。共鉴定出 2468 个 circRNA，其中 22 个在 RL 和 WL 组中差异表达（DE）。DE circRNA 宿主基因富集在卵巢甾体生成、MAPK 和 PI3K-Akt 信号通路中。此外，DE circRNA_0320 和 circRNA_0185 与 miR-143-3p 相互作用，miR-143-3p 是卵泡刺激素受体的靶标，对 GC 分化和卵泡发育至关重要。这些发现将有助于进一步分析在单色光照下导致母鸡 GC 发育的分子机制，从而提高产蛋量。
