Witkowski J A
Muscle Nerve. 1986 May;9(4):283-98. doi: 10.1002/mus.880090402.
This review continues with studies of protein, lipid, and purine metabolism of Duchenne muscular dystrophy (DMD) cells in vitro and of muscle cells in combined culture with nerve cells. In vitro studies of human metabolic myopathies are tabulated. Results using the hamster, chicken, and mouse (dy25, dy, mdg, and mdx) myopathies are discussed. Interesting findings include suggestions of altered collagen synthesis by DMD cells. Analysis of cell proteins by two-dimensional gel electrophoresis and the use of combined nerve-muscle cultures remain important areas of development. It is disappointing that so few attempts have been made to repeat significant findings in this field, and when a number of laboratories have examined the same phenomenon, the results are often contradictory. It remains to be shown how these various abnormalities found in cells in vitro are related to each other and to those pathologic features of diseased muscle observed in vivo.
本综述继续探讨杜兴氏肌营养不良症（DMD）细胞在体外的蛋白质、脂质和嘌呤代谢，以及与神经细胞联合培养的肌肉细胞的相关研究。对人类代谢性肌病的体外研究进行了列表整理。讨论了使用仓鼠、鸡和小鼠（dy25、dy、mdg和mdx）肌病模型的研究结果。有趣的发现包括DMD细胞中胶原蛋白合成改变的相关迹象。通过二维凝胶电泳分析细胞蛋白质以及使用神经 - 肌肉联合培养仍然是重要的发展领域。令人失望的是，在该领域重复重要发现的尝试极少，而且当多个实验室研究同一现象时，结果往往相互矛盾。体外细胞中发现的这些各种异常如何相互关联以及与体内患病肌肉的病理特征之间的关系仍有待阐明。
