Dobrzyński Maciej, Nikodem Anna, Klećkowska-Nawrot Joanna, Goździewska-Harłajczuk Karolina, Janeczek Maciej, Styczyńska Marzena, Kuropka Piotr
Department of Pediatric Dentistry and Preclinical Dentistry, Wroclaw Medical University, Krakowska 26, 50-425 Wroclaw, Poland.
Department of Mechanics, Materials and Biomedical Engineering, Faculty of Mechanical Engineering, Wrocław University of Science and Technology, Wybrzeże Wyspiańskiego 27, 50-370 Wroclaw, Poland.
Animals (Basel). 2022 Feb 16;12(4):484. doi: 10.3390/ani12040484.
The studies conducted so far indicate a negative effect of dioxins on the structure of the alveolar bone and teeth, especially in the developmental period in rats. The research aimed to analyze the indirect effect of dioxins contained in the body of female rats on the structure of the dental organ in their offspring in the neonatal period and to determine the possibility of reducing potential dioxin disorders of the structure of hard tissues in the offspring of intoxicated mothers by simultaneous administration of vitamin E or acetylsalicylic acid (ASA). Another goal of the research was to determine the level of magnesium, calcium and phosphorus contained in bone tissue as indicators of the mineralization process of hard tissues in rats, in the case of using 2,3,7,8-tetrachlorodibenzo--dioxin (TCDD) and acetylsalicylic acid or α-tocopherol. The experiment was carried out on eight female rats of the strain divided into four groups. From the offspring of eight females, the mandibles were removed from the mandibular joints, and then, after the removal of soft tissues, they were prepared for individual tests. Selected morphological, chemical and physical parameters of the teeth of the offspring of female rats from the experimental groups were analyzed. The analysis showed the effect of vitamin E and ASA on the content of Mg, Ca and P. In combination with TCDD, vitamin E and ASA, they positively inhibit the inflammatory process, preventing the leaching of Ca and Mg from the bones. ASA counteracted this phenomenon much more effectively than vitamin E. Detailed analysis of the tooth morphology showed that the molars' crowns exhibit shape disturbances under the influence of TCDD. Individual nodules in teeth T1, T2, T3 did not fuse, and the roots showed signs of hypertrophy. The study confirmed the negative effect of TCDD on tooth development. Teeth arising early in development are the most sensitive to the disorders, while the later ones are less exposed to the toxic effects of TCDD transmitted by the mother.
迄今为止进行的研究表明，二噁英对牙槽骨和牙齿结构有负面影响，尤其是在大鼠的发育阶段。该研究旨在分析雌性大鼠体内所含二噁英对其新生后代牙齿器官结构的间接影响，并确定通过同时给予维生素E或乙酰水杨酸（ASA）来降低中毒母亲后代硬组织结构潜在二噁英紊乱的可能性。该研究的另一个目标是确定在使用2,3,7,8-四氯二苯并-对-二噁英（TCDD）和乙酰水杨酸或α-生育酚的情况下，大鼠骨组织中镁、钙和磷的含量，作为硬组织矿化过程的指标。实验在8只该品系的雌性大鼠上进行，分为四组。从8只雌性大鼠的后代中，将下颌骨从下颌关节处取下，然后在去除软组织后，准备进行个体测试。对实验组雌性大鼠后代牙齿的选定形态、化学和物理参数进行了分析。分析表明维生素E和ASA对镁、钙和磷含量有影响。与TCDD、维生素E和ASA联合使用时，它们能积极抑制炎症过程，防止钙和镁从骨骼中流失。ASA比维生素E更有效地抵消了这种现象。对牙齿形态的详细分析表明，在TCDD的影响下，磨牙的牙冠出现形状紊乱。牙齿T1、T2、T3中的单个结节未融合，牙根显示出肥大的迹象。该研究证实了TCDD对牙齿发育的负面影响。发育早期长出的牙齿对这些紊乱最为敏感，而后期长出的牙齿较少受到母亲传递的TCDD毒性影响。
