Department of Entomology, University of Georgia, Athens, GA 30602, USA.
Department of Entomology, University of Georgia, Griffin, GA 30223, USA.
Cells. 2022 Feb 16;11(4):693. doi: 10.3390/cells11040693.
This review provides a synopsis of transcriptional responses pertaining to interactions between plant viruses and the insect vectors that transmit them in diverse modes. In the process, it attempts to catalog differential gene expression pertinent to virus-vector interactions in vectors such as virus reception, virus cell entry, virus tissue tropism, virus multiplication, and vector immune responses. Whiteflies, leafhoppers, planthoppers, and thrips are the main insect groups reviewed, along with aphids and leaf beetles. Much of the focus on gene expression pertinent to vector-virus interactions has centered around whole-body RNA extraction, whereas data on virus-induced tissue-specific gene expression in vectors is limited. This review compares transcriptional responses in different insect groups following the acquisition of non-persistent, semi-persistent, and persistent (non-propagative and propagative) plant viruses and identifies parallels and divergences in gene expression patterns. Understanding virus-induced changes in vectors at a transcriptional level can aid in the identification of candidate genes for targeting with RNAi and/or CRISPR editing in insect vectors for management approaches.
这篇综述概述了与植物病毒和以不同方式传播它们的昆虫媒介之间相互作用相关的转录反应。在这个过程中，它试图列出与媒介中病毒-媒介相互作用相关的差异基因表达，如病毒接收、病毒细胞进入、病毒组织嗜性、病毒复制和媒介免疫反应。本文综述了主要的昆虫群体，如粉虱、叶蝉、飞虱和蓟马，以及蚜虫和叶甲。与媒介-病毒相互作用相关的基因表达的大部分焦点都集中在全身 RNA 提取上，而关于媒介中诱导的组织特异性基因表达的数据有限。本综述比较了不同昆虫群体在获得非持久、半持久和持久（非增殖和增殖）植物病毒后的转录反应，并确定了基因表达模式的相似性和差异。在转录水平上了解病毒对媒介的诱导变化有助于识别 RNAi 和/或 CRISPR 编辑的候选基因，以用于昆虫媒介的管理方法。
