Huttala Outi, Loreth Desiree, Staff Synnöve, Tanner Minna, Wikman Harriet, Ylikomi Timo
Cell Biology, Faculty of Medicine and Health Technology, Tampere University, 33100 Tampere, Finland.
Tays Cancer Center, Tampere University Hospital, 33520 Tampere, Finland.
Biomedicines. 2022 Jan 26;10(2):271. doi: 10.3390/biomedicines10020271.
Vascularization plays an important role in the microenvironment of the tumor. Therefore, it should be a key element to be considered in the development of in vitro cancer assays. In this study, we decellularized in vitro capillaries to remove genetic material and optimized the medium used to increase the robustness and versatility of applications. The growth pattern and drug responses of cancer cell lines and patient-derived primary cells were studied on decellularized capillaries. Interestingly, two distinct growth patterns were seen when cancer cells were grown on decellularized capillaries: "network" and "cluster". Network formation correlated with the metastatic properties of the cells and cluster formation was observed in non-metastatic cells. Drug responses of patient-derived cells correlated better with clinical findings when cells were cultured on decellularized capillaries compared with those cultured on plastic. Decellularized capillaries provide a novel method for cancer cell culture applications. It bridges the gap between complex 3D culture methods and traditional 2D culture methods by providing the ease and robustness of 2D culture as well as an in vivo-like microenvironment and scaffolding for 3D cultures.
血管生成在肿瘤微环境中起着重要作用。因此，它应是体外癌症检测方法开发中需考虑的关键因素。在本研究中，我们对体外毛细血管进行脱细胞处理以去除遗传物质，并优化了培养基，以提高应用的稳健性和通用性。我们研究了癌细胞系和患者来源的原代细胞在脱细胞毛细血管上的生长模式和药物反应。有趣的是，当癌细胞在脱细胞毛细血管上生长时，观察到两种不同的生长模式：“网络状”和“簇状”。网络状形成与细胞的转移特性相关，而非转移性细胞则形成簇状。与在塑料上培养的细胞相比，当患者来源的细胞在脱细胞毛细血管上培养时，其药物反应与临床结果的相关性更好。脱细胞毛细血管为癌细胞培养应用提供了一种新方法。它通过提供二维培养的简便性和稳健性以及为三维培养提供类似体内的微环境和支架，弥合了复杂的三维培养方法与传统二维培养方法之间的差距。
